人群外周血组蛋白乙酰化在氟骨症发生中的作用  

Role of global histone H3 acetylation in patients with skeletal fluorosis

作　　者：范智鹏[1] 孙静[2] 刘洋[1] 李丙云[1] 郦芒[1] 刘晓娜[1] 孙殿军[1] 高彦辉[1] 

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心地氟病防治研究所国家卫生计生委病因流行病学重点实验室、黑龙江省普通高校病因流行病学重点实验室,哈尔滨150081 [2]宁夏回族自治区疾病预防控制中心地方病科,银川750004

出　　处：《中华地方病学杂志》2016年第3期174-177,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金重点项目（81172605）

摘　　要：目的探索高氟暴露是否会影响外周血组蛋白乙酰化水平，氟骨症患者的组蛋白乙酰化水平是否存在异常，尝试从转录修饰水平上研究氟骨症的发病机制。方法2012年，在青海省果洛州藏族居住地区和新疆维吾尔自治区阿勒泰哈萨克族居住地区，每个地区中选择2个县，并将每个县人口相对集中的3～4个饮茶型氟中毒病区乡确定为调查点。在病区按照年龄（相差不超过3岁）和民族匹配原则，选取59例典型成人氟骨症患者和59例健康人作为对照，共118人。对调查对象进行问卷调查，同时采集饮用茶水（或酥油茶）样、即时尿样和2mL全血。采用便携式数字化x光机拍摄前臂、腰椎及骨盆关节X线片用于病情诊断：采用氟离子选择电极法检测茶水氟浓度和尿氟浓度：采用比色法检测全血样本组蛋白H3乙酰化水平。结果人群外周血组蛋白H3乙酰化水平不同性别（男性：19．94mg／g；女性：20．05mg／g）和民族（藏族：17．73mg／g；哈萨克族：20．49mg／g）比较差异无统计学意义（U=1664．5、-0．902，P均〉0．05），但会随着年龄的升高而下降（r=-0．213，P〈0．05）。根据调查对象的日均茶水氟摄入量（0—3．25、〉3．25～8．05、〉8．05～12．33、〉12．33mg）和尿氟浓度（0-1．69、〉1．69—2．64、〉2．64～4．00、〉4．00mg／L）的四分位数分为4组，不同程度的茶氟暴露组和尿氟暴露组人群外周血组蛋白H3乙酰化水平（21．12、20．05、19．94、35．0，24．42、18．13、19．80、28．90mg／g）比较差异无统计学意义（H=1．706、5．928，P均〉0．05）。组蛋白H3乙酰化水平在对照组（19．43mg／g）和病例组（21．69mg／g）比较差异无统计学意义（Z=-1．39，P〉0．05）。不同程度病情人群（正常组、轻度组、中度组）外周血组蛋白H3乙酰化水平（中位数分别为19．43、21.32、35．95mg／g）比�Objective To explore the relationships between different levels of fluoride, the global histone H3 acetylation in peripheral blood mononuclear cells （PBMC） and the condition of skeletal fluorosis, which might be helpful in explaining the mechanism of skeletal fluorosis. Methods In 2012, two counties were chosen in each area, the Tibetan living area in Guoluo of Qinghai Province and the Kazakh living area in Ahay of Xinjiang Uygur Autonomous Region and 3 to 4 drinking brick-tea type fluorosis districts were chosen as the investigated districts in the counties. Totally 59 patients with skeletal fluorosis in the typical brick-tea type fluorosis districts were selected, and 59 healthy controls were selected based on age （difference ≤ 3 years old） and nationality. The adults were investigated by questionnaire, brick-tea water （or buttered tea）, urine and 2 ml peripheral blood samples were collected, and their skeletal fiuorosis was diagnosed according to the national criteria. The fluoride concentrations in brick-tea water （or Buttered tea） and urine were detected by fluoride ion selective electrode. The global histone H3 acetylation was quantified using colorimetric methord. Results The global histone H3 acetylation in PBMC between female and male population （female： 20.05 mg/g and male： 19.94 mg/g） was not significantly different （U = 1 664.5, P 〉 0.05）. The global histone H3 acetylation in PBMC between Tibetans （17.73 mg/g） and Kazakans （20.49 mg/g） was not significantly different （U = - 0.902, P 〉 0.05）. However, global histone H3 acetylation and age was positively correlated （r = - 0.213, P 〈 0.05）. There was no significant difference between tea fluoride （4 group： 0 - 3.25, 〉 3.25 - 8.05, 〉 8.05 - 12.33, 〉 12.33 mg） and global histone H3 acetylation in PBMC （21.12, 20.05, 19.94 and 35.04 mg/g, H = 1.706, P 〉 0.05）. There was also no significant difference between urine fluoride （4 group： 0 - 1.69, 〉 1.69 - 2.64, 〉 2.64 - 4.00, 〉 4.0

关 键 词：氟中毒 骨 组蛋白 乙酰化 

分 类 号：R599.1[医药卫生—内科学]