慢性氟中毒大鼠大脑和血清氧化应激水平改变及维生素E的拮抗作用  

Levels of oxidative stress in brain and serum of rats with chronic fluorosis and antagonistic effects ofVitamin E

作　　者：董阳婷 王娅[1] 魏娜[1] 官志忠[2] 

机构地区：[1]贵州医科大学病理学教研室,贵阳550004 [2]贵州医科大学分子生物学重点实验室、附属医院病理科

出　　处：《中华地方病学杂志》2016年第3期178-181,共4页Chinese Journal of Endemiology

基　　金：国家自然科学基金（81160335）;科技部支撑计划（2013BA105803）;贵州省科技厅国际合作项目（黔科合外G字[2011]7014号）

摘　　要：目的观察慢性氟中毒大鼠大脑及血清氧化应激水平改变及维生素E（VitaminE，VE）的拮抗作用，探讨慢性氟中毒脑损伤机制。方法健康纯系SD大鼠30只，按体质量采用随机数字表法分为3组，每组10只，雌雄各半。对照组自由饮用自来水，含氟量〈0．5mg／L；染氟组饮水中加入氟化钠，含氟量为50．0mg／L；染氟-VE拮抗组饮水含氟量为50．0mg／L，同时给予VE50．0mg／kg灌胃，每天1次。各组大鼠均给予标准饲料，含氟量6．2mg／kg。实验周期为10个月，处死大鼠，氟离子选择电极法测定骨氟含量；取脑组织和血清，黄嘌呤氧化酶法测定超氧化物歧化酶（SOD）活性、比色法测定谷胱甘肽过氧化物酶（GSH．Px）活性、硫代巴比妥酸荧光法测定丙二醛（MDA）含量以及比色法测定脑组织匀浆中羟自由基（OH-）、过氧化氢（H：02）、超氧阴离子自由基（咙）水平。结果染氟组大鼠骨氟含量明显高于对照组[（211．07±48．52）比（33．40±9．26）mg／kg，P〈0．01]。染氟组大鼠脑组织中SOD及GSH-P）【活性[（20．10±1．98）kU／g、（28．70±19．35）kU／L]均低于对照组[（37．05±3．13）kU／g、（59．63±12．83）kU／L，P均〈0．01]，而染氟．VE拮抗组SOD活性[（26．27±1．74）kU／g]高于染氟组（P〈0．01）；染氟组大鼠血清SOD及GSH—Px活性[（11．55±1．75）kU／L、（79．50±19．18）U／L]均低于对照组[（20．79±2．43）kU／L、（170．00±14．68）U／L，P均〈0．01]，而染氟一VE拮抗组SOD活性[（17．23±0．68）kU／L]高于染氟组（P〈0．01）；染氟组大鼠脑组织及血清MDA含量[（8．84±0．69）μmol／L、（1．46±0．11）nmol／L]均高于对照组[（1．27±0．74）μmol／L、（0．834-0．10）nmolL，P均〈0．01]，而染氟．VE拮抗组MDA含量[（4．51±1．13）μmol／L、（1．29±0．02）nmol／L]均低于染氟组（P均〈0．01）；染氟组Objective To detect the levels of oxidative stress in brain and serum of rats with chronic fluorosis and the antagonistic effects of vitamin E （VitE）, and to reveal the role of oxidative stress in brain injury. Methods Thirty healthy SD rats were divided into three groups based on body weight by means of a random number table （10 rats in each group, half male and half female）. In the control group, the rats were fed with drinking water containing less than 0.5 mg/L fluoride; in the fluoride group, the rats were fed with high doses of sodium fluoride in drinking water （50.0 mg/L） and the VitE antagonistic group were fed with the same content of fluoride in drinking water as the fluoride group, but adding VitE （50.0 mg/kg） by intragastric administration once a day. All rats were fed with normal diet （6.2 mg/kg）. After exposure to fluoride for 10 months, all rats were put to death, dental fluorosis of the rats was examined and the fluoride content in bone was determined by fluoride-ion selective electrode; the activity of superoxide dismutase （SOD） was determined by the xanthine oxidase method and glutathione peroxidase （GSH-Px） by the colorimetric method, the level of malonaldehyde （MDA） by the glucosinolates barbituric acid fluorescence method and the levels o f OH-, H2O2 and O2 in rat serum and/or brain were detected by the i colorimetric method. Results In the rats of the fluoride group, fluoride content in bone was higher as compared to control [bone fluoride： （211.07 ± 48.52） vs. （33.40 ± 9.26） mg/kg, P 〈 0.01]. The activities of SOD and GSH-Px in rat brains of the fluoride group [（20.10 ± 1.98） kU/g, （28.70 ± 19.35） kU/L] were significantly lower than those of controls [（37.05 ± 3.13） kU/g, （59.63 ± 12.83） kU/L, all P 〈 0.01], the activity of SOD in VitE antagonistic group [（26.27 ± 1.74） kU/g] was higher than the fluoride group （P 〈 0.01）; the activities of SOD and GSH-Px in rat serum of the fluoride group were significantly decr

关 键 词：氟中毒 氧化应激 脑 血清 维生素E 

分 类 号：R599.1[医药卫生—内科学]