2013年云南省H9N2亚型禽流感病毒两个进化分支毒株抗原性变异分析  

Antigenic Variation Analysis of Two Evolutionary Branches of H9N2 Subtype Avian Influenza Viruses from Yunnan Province in 2013

作　　者：石凤海 胡媛媛[2] 张文东[3] 孔强[4] 张应国[3] 范泉水[5] 赵焕云[3] 张富强[5] 

机构地区：[1]临沧市动物疫病预防控制中心,云南临沧677000 [2]云南农业职业技术学院,云南昆明650031 [3]云南省动物疫病预防控制中心,云南昆明650201 [4]云南农业大学,云南昆明650223 [5]成都军区疾病预防控制中心,云南昆明650032

出　　处：《动物医学进展》2016年第4期6-11,共6页Progress In Veterinary Medicine

基　　金：云南省科技计划项目(2012CH002);国家公益性行业专项(201103008);军队科技计划项目(CWS12J075;13BJYZ37;A12005)

摘　　要：为明确2013年云南省H9N2亚型禽流感病毒Ⅲ-1和Ⅲ-2小分支间是否存在抗原性差异,以及这种抗原性差异是否与氨基酸位点变异有关,从2个小分支中选取7个毒株与疫苗株进行系统发育分析和血清学交叉比对试验。结果表明,2个小分支HA基因核苷酸序列同源性为86.6%~98.4%,与2000年前疫苗株亲缘关系较远,Ⅲ-1分支与2000年后疫苗株（ACKLGQ113和ACKDL2010）亲缘关系较近。血清学交叉比对试验结果经统计学分析显示,Ⅲ-1和Ⅲ-2小分支间抗原性存在差别。氨基酸比对结果表明,不同进化亚分支毒株与疫苗株相比氨基酸存在变异;与已报道的抗原表位关键性位点相比,Ⅲ-1分支中部分毒株抗原表位关键性位点存在变异,Ⅲ-2分支毒株无变异,但在其他8个氨基酸位点存在特有变异,是否存在未知抗原表位关键性位点,以及其他氨基酸位点变异是否会辅助性影响抗原变异,还有待进一步研究。In order to investigate the antigenic differences between Ⅲ-1 and Ⅲ-2 of the H9N2 subtype AIV in Yunnan province in 2013, and whether the antigenic difference is related to the amino acid mutation, 7 vi- rus strains and vaccine strains were analyzed by phylogenetic analysis and serological cross test. The results showed that：the homologies of HA gene of the two subgroup strains were 86.6% to 98.4%, the relationship was far with the vaccine strains before 2000, Ⅲ-1 strains were near with the vaccine strains （ACK- LGQll3 and ACKDL2010）after 2000. The HI cross test results by statistics analysis showed that the anti- genicity differences exist between Ⅲ-1 and Ⅲ-2 subgroups. Amino acid comparative analysis result showed that variations in different subgroup strains exist compared with the antigenic epitope sites reported, the variation existed in parts of Ⅲ-1 strains and in Ⅲ-2 strains is no change,but there are unique variations in eight other amino acid sites of Ⅲ-2 strains, whether there is any unknown antigen epitope sites, or the other ancillary amino acid sites whether can affect antigen variation ,have yet to be further studied.

关 键 词：禽流感病毒 H9N2亚型 进化分支 抗原性变异分析 

分 类 号：S852.695.2[农业科学—基础兽医学]