羊脑泡状棘球蚴病实验模型建立可行性探讨  

The feasibility investigation of experimental animal model of cerebral alveolar echinococcosis disease of sheep

作　　者：姚卫宏[1] 张泉[1] 王俭[1] 姜涛[2] 姜春晖[1] 张德清[1] 马娟[1] 吴玲[1] 

机构地区：[1]新疆医科大学第一附属医院影像中心磁共振室,新疆乌鲁木齐830011 [2]新疆医科大学第一附属医院动物研究中心,新疆乌鲁木齐830011

出　　处：《实用放射学杂志》2016年第3期448-451,共4页Journal of Practical Radiology

基　　金：国家自然科学基金项目（30860268,81560279）;新疆维吾尔自治区自然科学基金项目（2011211A072）;乌鲁木齐高新技术产业开发区（新市区）科学技术计划项目（Y2012024）.

摘　　要：目的探讨建立羊脑泡状棘球蚴（AE）病实验模型的方法，以利于人类脑AE病的相关研究。方法选择实验用健康新疆大尾羊10只，直视下颅骨穿刺，脑内接种AE源头蚴，8个月后用MRI观察羊脑AE生长状况，剖检后分析形态学及病理学特点。结果经病理及MRI证实，成功建模4例，成虫率40％（4／10），4例均为单发病灶，1例影像学表现具有特征性，T2WI表现为低信号背景下多发小囊泡群，3例T2WI表现为低信号，光镜下观察病灶区有大量淋巴细胞、嗜酸性粒细胞及浆细胞浸润，周围小血管闭塞并伴有炎性反应。结论人工接种鼠源性AE原头蚴悬液建立羊脑AE病模型可行性高，方法简单，具有可重复性。Objective To build experimental animal model of cerebral alveolar echinococcosis disease of sheep,in order to study of human alveolar hydatid disease of the brain. Methods Experiment animal models of ten Xinjiang big tail sheep were performed by, direct skull puncture,intracerebral inoculation of echinococcus multilocularis. MRI was used to observe the growth status of cerebral alveolar echinococcosis disease of sheep after 8 months, and morphological and pathological characteristics after autopsy were ana lysed. Results 4 sheep models （40%） were successful built which were confirmed by pathology and MRI. On MRI,4 cases all were single lesion,on T2WI there was multiple follicles bubbles under the background of low signal in one case, and low signal in other three cases. Under the microscope, a large number of lymphocytes, eosinophils and plasma ceils infiltrated the lesion area, around which small blood vessels were blocked and had inflammatory reaction were showed. Conclusion The method using artificial inocula tion rat alveolar eehinococeosis to establish experimental model of cerebral alveolar eehinocoecosis disease of sheep has the character- istic of feasibility, simplicity and repeatability.

关 键 词：脑泡状棘球蚴病 新疆绵羊 动物模型 磁共振成像 

分 类 号：R532.32[医药卫生—内科学] R-332[医药卫生—临床医学]