透明质酸抑制下颌骨髁突软骨钙化的实验研究  

Hyaluronic acid inhibites the calcification of mandibular condyle

作　　者：赵天一 蔺栋鹏 田海锁 贾骏麒 张武阳 马秦 

机构地区：[1]军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病临床医学研究中心,第四军医大学口腔医院颌面外科,西安710032 [2]河南省人民医院口腔科 [3]武警辽宁省总队医院口腔科

出　　处：《实用口腔医学杂志》2016年第2期167-172,共6页Journal of Practical Stomatology

摘　　要：目的:探究透明质酸(HA)对下颌骨髁突软骨钙化的影响及其机制。方法:取新生小鼠(3 d)的髁突软骨及长骨软骨分别置于空白(培养对照组)和加有HA(HA组)的培养基中行小组织块培养,分别培养6周后,HE、茜素红、碱性磷酸酶(ALP)染色观察2组软骨组织的钙化情况,免疫组化观察VEGF、MMP-13蛋白表达情况。结果:培养对照组髁突软骨内观察到钙化、基质中VEGF和MMP-13表达阳性以及阳性细胞较多。HA组未观察到髁突和长骨软骨内成骨和钙化发生,但观察到软骨表面积的增大,VEGF及MMP-13在软骨细胞基质内表达阴性,阳性细胞率减少(P<0.05)。结论:在体外培养环境下,HA可以抑制下颌骨髁突软骨钙化,其机制可能是抑制了VEGF及MMP-13的表达。Objective： To study the effects of hyaluronic acid（ HA） on the calcification of mandibular condylar cartilage. Methods：Mandibular condylar cartilage and long bone cartilage were sampled from neonatal mice（ 3 d） and were randomly divided into medium control group and HA group. After 6 weeks of in vitro culture,the degree of calcification was evaluated by HE staining,Alizarin Red staining and ALP staining. The expression of VEGF and MMP-13 was detected by immunohistochemical staining. Results： In the medium control group calcification in cartilage was not found,VEGF and MMP-13 were expressed in the cartilage matrix and in some cells,the long bone cartilage surface area was increased（ P 〈0. 05）. In the HA treatment groups of condyl and long bone cartilage no calcification was found,increase of the cartilage surface area was observed; VEGF and MMP-13 were negative in the cartilage matrix was decreased,and the positive expression cells were reduced（ P 〈0. 05）. Conclusion： HA can inhibit calcification of mandibular condylar cartilage,which may be associated with the inhibition of VEGF and MMP-13 expression.

关 键 词：髁突软骨 组织培养 VEGF 钙化 MMP-13 透明质酸(HA) 

分 类 号：R684.3[医药卫生—骨科学]