石决明对氧化应激所致白内障的作用研究  

Experimental Study of the Abalone Shell on Cataract induced by Oxidative Stress

作　　者：周宇[1] 金鑫[1] 祁磊[2] 王玉斌[2] 徐清妍 李秀娟[2] 郑玛丽[2] 钟瑞生 林媛[2] 

机构地区：[1]厦门大学医学院基础医学部,厦门3611022 [2]福建省厦门市中医院眼科,厦门361009

出　　处：《光明中医》2016年第5期650-655,共6页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：2012年福建省卫生厅中医药科研专项课题(No.WST201210);2013年福建省卫生厅中医药科研专项课题(No.wzhw201302);2014年厦门市科技局科技惠民项目课题(No.3502Z20144030)

摘　　要：目的研究石决明提取物对体外培养的晶状体氧化应激性白内障形成的作用及机制。方法离体培养小鼠晶状体,应用不同浓度的石决明提取物预孵育晶状体24h后,加入1mm过氧化氢,继续培养3小时后恢复正常培养,72小时后观察小鼠晶状体混浊程度,测定晶状体组织培养液中的乳酸脱氢酶（LDH）含量,晶状体组织中还原型谷胱甘肽（GSH）含量及超氧化物歧化酶（SOD）活力。结果石决明提取物在1-2 mg/ml浓度范围内减轻氧化应激造成的晶状体混浊,减少晶状体LDH的释放,提高组织内GSH含量和SOD活力。结论石决明提取物可减轻氧化应激白内障的形成,其作用主要与石决明提取物提高内源性抗氧化系统有关。Objective To study the effect of the abalone shell extract on oxidative stress induced cataract formation and its mechanism in cultured mouse lens in vitro. Methods The cultured mouse lens were pretreated with the abalone shell extract in different concentrations for24 hours,and then 1mm hydrogen peroxide was added and continued incubating for 3 hours,and they were changed to normal culture media.After 72 hours,the opacity of each lens was observed under an anatomical microscope,the content of lactate dehydrogenase（ LDH）leakages,the content of the reduced glutathione（ GSH） and activity of superoxide dismutase（ SOD） in lens tissue were assayed. Results Abalone shell extract in the concentration range of 1 - 2 mg / ml reduced the lens opacity caused by oxidative stress,alleviated the release of LDH,and increased GSH content and SOD activity in cultured lens. Conclusion Abalone shell extract can alleviate the oxidative stress induced cataract formation,and this effect is mainly related to its improvement of the endogenous antioxidant system in lens.

关 键 词：石决明 白内障 晶状体 氧化应激 小鼠 

分 类 号：R285.5[医药卫生—中药学]