梨β–微管蛋白基因及其在矮生型与普通型梨茎尖中的表达差异  

A Group of β-tubulin Genes and Their Expression Difference in Stem Tips Between the Dwarf and Standard Type Pears

作　　者：侯董亮 王彩虹[1] 田义轲[1] 肖玉雄[1] 张蓓[1] 

机构地区：[1]青岛农业大学园艺学院,山东青岛266109

出　　处：《园艺学报》2016年第2期320-328,共9页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(31372049);山东省良种工程项目(2012-2015)

摘　　要：以模式植物拟南芥的β–微管蛋白为参考依据,通过电子克隆的方法,从西洋梨基因组中克隆了9个β–微管蛋白基因,并根据苹果和梨基因组间的保守性及共线性原理,实现了这些基因的染色体定位。序列分析显示,这组基因均含有3个外显子和两个内含子。起源于全基因组复制事件而形成的成对染色体上的基因不仅在外显子和内含子长度上具有较高的保守性,而且编码的氨基酸残基也有更高的序列相似性。对9个β–微管蛋白基因在矮生梨与普通梨杂种群体中的荧光定量表达分析表明,有7个基因在茎尖组织中表达,其中定位于chr16上的转录本号为PCP044487.1的基因在矮生型和普通型梨茎尖中的转录水平出现极显著差异,推测该基因可能与梨矮生性状形成的分子调控有重要关系。Using the β-tubulins of Arabidopsis as references,9 β-tubulin genes were identified from the Pyrus communis genome. These genes were mapped in the corresponding chromosomes based on the principle of conservation and collinearity between the genomes of apple and pear. Sequence analysis showed that each of these genes contain 3 exons and 2 introns. For the genes located in the pair of chromosomes which originated from the genome wide duplication in evolution,the lengths of exons and introns are more conserved,and their coding sequences of amino-acid residues are with higher similarity. The result of q RT-PCR showed that,7 out of the 9 β-tubulin genes expressed normally in the tissue of stem tips. Among them,the transcriptional level of the gene with the transcript number of PCP044487.1,mapped in chromosome 16,is significantly different at P ≤ 0.01 between the dwarf type and the standard type pears stem tips,which suggested that this gene probably related to molecular regulation of the dwarf trait formation.

关 键 词：梨 矮生性状 β–微管蛋白 基因结构 转录水平 

分 类 号：S661.2[农业科学—果树学]