正交设计优化精制冠心颗粒的破壁提取工艺  

Optimization of Breaking- Extraction Process for Jingzhi Guanxin Particle by Orthogonal Test

作　　者：韩雪[1] 林俊芝 郭治平 邹文铨[4] 韩丽[1] 杨明[5] 张定堃[1] 

机构地区：[1]成都中医药大学药学院,四川成都611137 [2]成都中医药大学附属医院,四川成都610072 [3]四川厚德医药科技有限公司,四川成都611731 [4]四川大学化学学院,四川成都610064 [5]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004

出　　处：《辽宁中医杂志》2016年第3期566-569,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81403115);四川省重大产业技术创新专项项目(2011ZY00299)

摘　　要：目的:针对传统回流提取易导致有效成分降解的弊端,研究破壁浸提精制冠心颗粒的最优工艺。方法:以芍药苷、阿魏酸与丹酚酸B含量的综合评分为评价指标,采用单因素结合正交试验考察溶媒用量、浸提次数、浸提温度与浸提时间对提取工艺的影响。结果:最佳提取工艺为药材粉碎至d0.5为14μm左右,加入8倍量水,在60℃条件下浸提3次,每次10 min。结论:该优选工艺稳定可行,能在提取环节显著增加有效成分的提取量,可代替传统的回流工艺。Objective： To optimize the breaking- extraction process of Jingzhi Guanxin Particle,in order to overcome the disadvantage of effective components degradation. Methods： With composite score of the contents of paeoniflorin,ferulic acid and salvianolic acid B as indexes,single factor test and orthogonal test were applied to optimize extraction with the extraction time,times,temperature and the amount of water as factors. Results： The best extraction conditions were as follows： d 0. 5 of herbs were ground into about 14 μm,extracted 3 times in the condition of 60 ℃ with 8- fold amount of water,10 min for each time. Conclusion： This optimized extraction process was reasonable and feasible,and could significantly increase the extraction amount of effective components,expecting to replace the conventional reflow process.

关 键 词：精制冠元颗粒 正交设计 破壁提取 热敏性成分 

分 类 号：R284[医药卫生—中药学]