阳离子树脂纯化川贝母总生物碱的工艺研究  

Technology Study on Total Alkaloids from Cation Resin Purification Fritillaria

作　　者：刘元涛[1] 戴兵[1] 邵珠德 高鹏[1] 代龙[1] 

机构地区：[1]山东中医药大学药学院,山东济南250355

出　　处：《辽宁中医杂志》2016年第3期583-586,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"重大新药创制"科技重大专项(2013ZX09103002-015)

摘　　要：目的:研究阳离子交换树脂分离纯化川贝母总生物碱的工艺条件。方法:以川贝母中西贝母碱、总生物碱的比吸附量、洗脱率、转移率为指标,优选川贝母中总生物碱的最佳纯化工艺。结果:最佳纯化工艺为川贝母酸水提取液以6 BV/h的流速逆向通过001×7氢型阳离子交换树脂柱(柱径与柱高比为1:2.5)进行动态吸附,树脂饱和后,水洗至中性,再用5 BV含5%氯化钠的60%乙醇液洗脱,洗脱速度为10 BV/h,收集洗脱液。川贝母中西贝母碱的洗脱率为75.44%,转移率为56.69%。得膏率为2.12%,总生物碱含量为74.31%。结论:采用001×7氢型阳离子交换树脂纯化西贝母碱效率高,成本低,在保证转移率的前提下能显著降低得膏率,实现对传统方剂中药物精制的目的。Objective： To study the process conditions of total alkaloids from cation resin purification Fritillaria. Methods： Taking the adsorption capacity of sipeimine and the total alkaloids,elution rate and the transfer rate as indicators,we optimized the best purification process of total alkaloids. Results： Best purification process： Fritillaria acidic water extract with 6 BV / h flow rate flowed to 001 × 7 reverse hydrogen type cation exchange resin column（ column diameter and column height ratio was 1： 2. 5） for dynamic adsorption. After resin saturation,we washed with water until to be neutral,and then 5% sodium chloride containing 5 BV of 60% ethanol solution with the elution rate of 10 BV / h,collecting the eluate. The sipeimine elution from Fritillaria was 75. 44% and the transfer rate 56. 69%. The extract yield rate was 2. 12% and the total alkaloid 74. 31%. Conclusion： The 001 × 7 cation exchange resin purification sipeimine has high efficiency and low cost,to ensure the transfer rate can significantly reduce the rate and achieve the traditional prescription drug refining purposes.

关 键 词：川贝母 西贝母碱 总生物碱 阳离子树脂 

分 类 号：R284[医药卫生—中药学]