巨噬细胞刺激蛋白在慢性阻塞性肺疾病患者中的表达及意义  

The Expression and Significance of Macrophage Stimulating Protein in Patients with Chronic Obstructive Pulmonary Disease

作　　者：张文波[1] 陈小菊[1] 冷长燕[1] 向小均 

机构地区：[1]川北医学院附属医院呼吸内科,四川南充637000 [2]重庆三峡中心医院急诊科,重庆万州404000

出　　处：《中国呼吸与危重监护杂志》2016年第2期115-118,共4页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：四川省卫生厅科研项目(编号:070293)

摘　　要：目的探讨巨噬细胞刺激蛋白(MSP)在慢性阻塞性肺疾病(简称慢阻肺)发病机制中的作用。方法收集2013年7月至2013年12月于川北医学院附属医院体检、门诊或住院患者,纳入健康对照者、慢阻肺稳定期和慢阻肺急性加重期患者各30例分成三组,采用酶联免疫吸附法检测三组受试者血浆中MSP、白细胞介素-6(IL-6)、IL-8、肿瘤坏死因子α(TNF-α)的浓度,以及慢阻肺急性加重期和稳定期组患者诱导痰中MSP的浓度。结果慢阻肺急性加重期和稳定期组患者血浆中MSP、IL-6、IL-8、TNF-α的浓度均明显高于健康对照组(P<0.05),而慢阻肺急性加重期组患者血浆中MSP、IL-6、IL-8、TNF-α的浓度又明显高于慢阻肺稳定期组(P<0.01)。慢阻肺急性加重期组患者诱导痰中MSP的浓度高于慢阻肺缓解期组(P<0.01)。慢阻肺患者血浆和诱导痰中MSP、血浆IL-6、IL-8、TNF-α的浓度水平与其FEV1占预计值百分比均呈负相关。慢阻肺患者血浆和诱导痰中MSP浓度水平与血浆中IL-6、IL-8、TNF-α的浓度水平均呈正相关。结论慢阻肺患者体内合成和释放MSP、IL-6、IL-8、TNF-α明显增多。慢阻肺患者血浆和诱导痰中MSP的浓度及慢阻肺患者血浆中IL-6、IL-8、TNF-α的浓度均与其气流受限严重程度相关。MSP在慢阻肺的发病机制中发挥重要作用,可能主要是参与气道炎症的调节。Objective To investigate the role of macrophage stimulating protein（ MSP） in the pathogenesis of chronic obstructive pulmonary disease（ COPD）. Methods Ninety subjects were recruited from health examination center,outpatient or inpatient department in Affiliated Hospital of North Sichuan Medical College from July 2013 to December 2013. They were divided into a healthy control group,a stable COPD group,and an acute exacerbation of COPD（ AECOPD） group with 30 subjects in each group. The levels of MSP,interleukin-6（ IL-6）,IL-8 and tumor necrosis factor-α（ TNF-α） in the plasma of all subjects,as well as the levels of MSP in the induced sputum of the AECOPD and the stable COPD patients were assessed by enzyme-linked-immuno-sorbent assay. Results The concentrations of MSP,IL-6,IL-8 and TNF-α in the plasma of the patients with COPD were obviously higher than those of the healthy controls（ P〈0. 05） while much higher in AECOPD patients than those in stable COPD patients（ P〈0. 01）. The concentration of MSP in the induced sputum of the patients with AECOPD was higher than that in the stable COPD patients（ P〈0. 01）. The concentrations of MSP in the serum and induced sputum as well as serum levels of IL-6,IL-8 and TNF-α in the patients with COPD were negatively correlated with the level of FEV1% pred. The concentrations of MSP in the serum and induced sputum in the COPD patients were positively correlated with the concentrations of IL-6,IL-8 and TNF-α. Conclusions The concentrations of serum and induced sputum MSP,and the serum concentrations of IL-6,IL-8 and TNF-α in COPD patients are related to the severity of the disease. MSP may play an important role in the pathogenesis of COPD. The mechanism might be mainly involved in the regulation of airway inflammation.

关 键 词：巨噬细胞刺激蛋白 慢性阻塞性肺疾病 白细胞介素6 白细胞介素8 肿瘤坏死因子Α 

分 类 号：R563.9[医药卫生—呼吸系统]