白细胞异常增加对精液质量及氧化应激的影响  

The impacts of leukocytes elevation on the sperm quality and oxidative stress

作　　者：张俏忻[1] 肖颖秀[2] 程碧珍[1] 

机构地区：[1]汕头大学医学院第一附属医院检验科,广东汕头515041 [2]汕头大学医学院第一附属医院神经内科,广东汕头515041

出　　处：《国际检验医学杂志》2016年第6期726-727,730,共3页International Journal of Laboratory Medicine

基　　金：广东省医学科研基金资助项目(A2014441)

摘　　要：目的研究白细胞(WBC)异常增加对精液质量及氧化应激的影响。方法共收集52份WBC异常增高精液标本和50份WBC正常精液标本,彩色精子自动分析系统分析精液常规,以鲁米诺为探针,运用化学发光法检测精子活性氧(ROS)水平。分别采用硫代巴比妥酸法和黄嘌呤氧化酶法检测精液中丙二醛(MDA)和总超氧化物歧化酶(T-SOD)的水平。结果 WBC异常组和WBC正常组的精液常规参数中总活力、前向运动力和正常精子比率与WBC正常组之间差异具有统计学意义(P=0.008;P=0.005;P=0.003);精子运动参数中曲线速度(VCL)(P=0.023)、直线速度(VSL)(P=0.010)、平均路径速度(VAP)(P=0.011)、摆动性(WOB)差异具有统计学意义(P<0.05);精子形态异常率差异具有统计学意义(P=0.008)。WBC异常组较WBC正常组精液标本中ROS、MDA显著增加,差异具有统计学意义(P<0.05),而T-SOD在两组之间差异无统计学意义(P>0.05)。结论 WBC异常增高可引起精子质量下降,其机制可能与WBC增加释放过多的活性氧有关。Objective To study the impacts of leukocytes elevation on the sperm quality and oxidative stress.Methods A total of 52 elevated leukocytes specimen and 50 normal leukocytes specimen were collected.Standard semen analysis was performed.Reactive oxygen species（ROS）production was measured using luminol as the probe by the Chemiluminescence method.Malonaldehyde（MDA）content in semen was measured by the sulfo-barbitone acid method.Total superoxide dismutase（T-SOD）in semen was measured by the xanthine oxidase method.Results The semen parameters including total motility（P=0.008）,progressive motility（P=0.005）and normal forms（P=0.003）,the sperm motion parameters including mean curvilinear velocity（VCL）（P=0.023）,mean straight-line velocity（VSL）（P=0.010）,mean average path（VAP）（P=0.011）,swing（WOB）（P〈0.001）in the elevated leukocytes group all decreased significantly compared with the normal leukocytes group.The ratio of sperm abnormality in the abnormal leukocytes group increased significantly（P=0.008）.ROS levels（using the log-transformation）and Malondialdehyde（MDA）were significantly higher in the elevated leukocytes group,but no significant difference was observed for T-SOD between the two groups（P〉0.05）.Conclusion The leukocytes elevation could result in the decrease of spermatozoa quality,which might be related with ROS increase.

关 键 词：白细胞 精子 活性氧 

分 类 号：R446.19[医药卫生—诊断学]