医院分离耐碳青霉烯类药物鲍曼不动杆菌亲缘性的研究  

The research on affinity of hospital separation carbapenems-resistant Acinetobacter

作　　者：李光荣[1] 向成玉[1] 杨葵[1] 刘靳波[1] 

机构地区：[1]泸州医学院附属医院检验科,四川泸州646000

出　　处：《国际检验医学杂志》2016年第6期740-742,共3页International Journal of Laboratory Medicine

基　　金：泸州医学院附属医院基金资助项目(14035)

摘　　要：目的通过分析鲍曼不动杆菌(Ab)碳青霉烯类药物耐药和敏感菌株蛋白指纹图谱,了解碳青霉烯类药物耐药菌株亲缘性远近的临床应用价值。方法收集临床分离对碳青霉烯类药物耐药(22株)和敏感的Ab(18株),利用表面增强激光解析电离飞行时间质谱技术(SELDI-TOF-MS)检测细菌蛋白质指纹图谱。采用Biomarker Wizard 3.1软件分析碳青霉烯类药物耐药和敏感Ab相关差异蛋白,采用SPSS 19.0对差异蛋白进行聚类分析,追溯其临床感染的亲缘性。结果碳青霉烯类药物耐药与敏感的Ab菌株间蛋白质谱的表达差异有统计学意义(P<0.05)。聚类分析结果显示碳青霉烯类药物耐药Ab在院内的分布以A型为主,B型次之。结论聚类分析碳青霉烯类抗菌药物耐药的Ab蛋白质谱结果能够判断细菌亲缘关系远近,可为Ab感染和临床流行病学调查提供理论依据。Objective To study the protein fingerprinting of carbapenems resistant and susceptible Acinetobacter baumannii（Ab）strains,investigate the clinical value of affinity distance in carbapenems resistant strains.Methods A total of 22 carbapenems resistant Ab strains and 18 carbapenems susceptible Ab strains were collected,and bacterial protein fingerprinting was detected by surface-enhanced laser desorption ionization time of flight mass spectrometry（SELDI-TOF-MS）,differentially expressed proteins was analyzed by Biomarker Wizard 3.1.Cluster analysis of differential expressed proteins was conducted on SPSS 19.0.ResultsThe protein expression pattern of carbapenems resistant and sensitive Ab strains had significant difference（P〈0.05）.Cluster analysis showed carbapenems resistance Ab was given priority to with type A,followed by type B.Conclusion The results of cluster analysis carbapenems resistance of Ab protein fingerprinting could determine the distance of affinity relationship of Ab.It could provide a theoretical basis for the infection and clinical epidemiology of Ab.

关 键 词：鲍曼不动杆菌 表面增强激光解析电离飞行时间质谱技术 聚类分析 蛋白组学 

分 类 号：R446.5[医药卫生—诊断学]