美国山核桃不定根形成过程中相关蛋白质的鉴定及功能分析  

Identification and function prediction of proteins during the adventitious rooting of pecan hardwood cuttings

作　　者：曹凡[1] 彭方仁[1] 梁有旺[1] 谭鹏鹏[1] 王克春[1] 吕昕[1] 

机构地区：[1]南京林业大学林学院,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2016年第2期53-58,共6页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业公益性行业科研专项项目(201304711);江苏省林业三新工程项目[Lysx2014(047)];江苏高校优势学科建设工程资助项目(PAPD)

摘　　要：美国山核桃是扦插后极难生根的树种,为研究其不定根生根机制,利用MALDI-TOF-MS技术对美国山核桃不定根发育关键时期的差异蛋白进行鉴定分析,发现了48个表达相对差异的蛋白点,选择其中18个差异蛋白进行肽质谱指纹分析并进行同源性比较。结果表明,美国山核桃不定根形成的生理过程中产生了差异蛋白质,其中包括了能量代谢相关蛋白、逆境胁迫相关蛋白和信号传递相关蛋白等差异蛋白。分析可知:能量代谢相关蛋白有ATP合成相关酶类、光合作用相关蛋白及烯醇化酶等;逆境胁迫相关蛋白与热激蛋白同源;信号传递相关蛋白主要为细胞色素酶类和血红结合蛋白。The expressed proteins during the adventitious rooting of Carya illinoinensis （pecan） hardwood cutting were i- dentified and analyzed by MALDI-TOF-MS to evaluate its rooting mechanism. We found 48 proteins expressed differen- tially during adventitious rooting of Carya illinoinensis （pecan） hardwood cutting, 18 proteins of which were analyzed by peptide mass fingerprinting homology. The results showed that the different expressed proteins included energy metabolism proteins, adversity stress proteins and signal transmission proteins.Energy metabolism related proteins were ATP synthase, photosynthesis related proteins and enolase, etc.. Adversity stress proteins contained heat shock-related protein. Signal transmission proteins were mainly cytochrome enzymes and heme-binding protein.

关 键 词：美国山核桃 不定根形成 特异性蛋白 双向电泳 

分 类 号：Q786[生物学—分子生物学] S718[农业科学—林学]