日本乙型脑炎病毒对猪外周血单核细胞炎性因子mRNA转录时相影响的研究  

Study on effect of Japanese encephalitis virus on mRNA transcriptional profiles of related inflammatory factor in porcine peripheral blood mononuclear cells

作　　者：何潇湘[1] 景亚星 靳晓慧[3] 兰培英[1] 张利卫[3] 周雍[1] 魏战勇[1,3] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002 [3]河南省动物性食品安全重点实验室,河南郑州450002

出　　处：《河南农业大学学报》2016年第1期60-65,共6页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(U1404326);河南省科技开放合作项目(142106000042)

摘　　要：为分析猪日本乙型脑炎病毒(JEV)感染猪外周血单核细胞后对其促炎因子转录的影响,探讨JEV感染的分子免疫致病机制。采用real-time RT-PCR方法,测定并分析了JEV感染猪外周血单核细胞(PBMC)后病毒RNA含量及白细胞介素(IL-8、IL-12p35、IL-12p40、IL-17、IL-18)炎性细胞因子mRNA转录时相的变化。结果显示,JEV感染猪PBMC后引起IL-8,IL-12p35和p40的分泌呈现不规则的曲线,IL-18表达量持续升高;IL-17表达量持续下降,仅在感染后48 h的时候达到峰值。以上结果表明,JEV感染PBMC可促使炎性因子分泌增加,参与细胞免疫应答。In order to analyze the influence on the mRNA expression level of pro-inflammatory cytokine in porcine peripheral blood mononnclear cells （PBMCs） after the Japanese encephalitis virus （JEV） infection, and to explore the molecular mechanism of JEV infection, the quantitative real-time RT-PCR method was used to determine and analyze the RNA of JEV that infected in PBMC. The transcript levels of the inflammatory eytokines （ IL-8, IL-12p35 ,IL-12p40, IL-17, IL-18） were also determined. The result indicated that JEV could infect the PBMC. The transcript level of IL-8, IL-12p35 and IL-12p40 rendered irregular curves, and IL-18 continued to rise, while IL-17 keep dropping, reaching its peaks 48 h after infection. These results showed that JEV infected in PBMC may contribute to increase the secretion of inflammatory factors, and then gets involved in cell-mediated immune response.

关 键 词：日本乙型脑炎病毒 猪外周血细胞 炎性细胞因子 转录时相 

分 类 号：S828[农业科学—畜牧学]