外源亚精胺对鸭梨自花授粉花粉管伸长及花柱蛋白表达的影响  

Effects of Exogenous Spd on the Pollen Tube Growth and Stylar Protein Expression During Pollination and Fertilization of Yali Pear

作　　者：岳雷[1,2] 李中勇[1] 尹宝颖[1] 张媛[1] 徐继忠[1] 

机构地区：[1]河北农业大学园艺学院,河北保定071001 [2]沧州市农林科学院,河北沧州061001

出　　处：《华北农学报》2016年第1期22-28,共7页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目(C200600429)

摘　　要：为探讨外源多胺调控鸭梨自交不亲和的生理生化机制,分别以自花授粉和外源亚精胺处理后1~3 d的花柱为试材,测定了不同处理不同时期花粉管生长发育动态,利用双向电泳技术和质谱分析技术分析了外源亚精胺处理前后不同时期鸭梨花柱蛋白的差异表达。结果显示：与对照相比,不同浓度的Spd处理均能极显著促进花粉管生长,0.25 mmol/L Spd处理72 h后可使大量花粉管到达花柱基部;在自交花柱电泳图谱中发现了3种特异蛋白质,A蛋白质Mr A=28.0 k Da,p I=5.7;B蛋白质Mr B=28.5 k Da,p I=5.7;C蛋白质Mr C=22.0 k Da,p I=5.3,这3种特异蛋白质可能与自交不亲和性相关;质谱分析获得了2个较好的蛋白点,分别为推定和假定蛋白。Yali pear belongs to the kind of gametophytic self-incompatibility,the percentage of self-pollinated fruit set is extremely low. Our previous research showed that sprayed polyamine in a concentration range at florescence could break self-incompatibility of Yali pear. In order to explore the physiological and biochemical mechanism,the effects of exogenous Spd on the pollen tube growth and stylar protein expression of the Yali pear were studied by two-dimensional gel electrophoresis and mass spectrum analysis technology. The main results were as follows：The pollen tube length were extremely significantly increased after suitable concentration of Spd treatment,the best effects of exogenous Spd were 0. 25 mmol / L Spd treatment,the number of pollen tube reached to the bottom of style were the most after 72 hours of treatment. Three specific proteins were found in the electrophoretogram. Two protein spots were obtained by the mass spectrometry results,which were predicted protein and putative protein respectively.

关 键 词：亚精胺 鸭梨 花粉管 花柱蛋白 

分 类 号：Q78[生物学—分子生物学] S661.03[农业科学—果树学]