新疆野苹果幼苗对盐胁迫的生理响应  

Physiological Responses of Malus sieversii Seedling Under Salinity Stress

作　　者：于玮玮[1] 曹波[1] 龙鸿[1] 李慧[1] 李爱[1] 阎国荣[1] 

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《华北农学报》2016年第1期170-174,共5页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金青年项目(31300564);新疆维吾尔自治区财政林业科技专项(400173);天津市科技特派员项目(14JCTPJC00497)

摘　　要：为了解新疆野苹果盐胁迫下的生理特性,以新疆野苹果实生幼苗为试材,设置6个Na Cl梯度(0,0.2%,0.4%,0.6%,0.8%,1.0%)处理4 h和0.4%Na Cl溶液处理7个时间梯度(0,4,8,12,16,20,24 h),采样测定其叶片中脯氨酸(Pro)、丙二醛(MDA)、叶绿素(Chl)和可溶性糖含量以及超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的变化。结果表明:叶片中脯氨酸的含量在Na Cl浓度为0.6%时和Na Cl浓度为0.4%处理12 h时,达到峰值,分别比对照提高了63.1%和27.0%。随着盐浓度的升高和盐胁迫时间的延长,丙二醛的含量呈现出逐渐增加的趋势。叶绿素变化呈现出先降低后升高再降低的趋势,所有处理中,叶绿素含量均低于正常水平。可溶性糖含量、SOD和POD的活性均呈现先上升到达峰值后再下降的趋势。新疆野苹果幼苗在盐胁迫下可通过积累脯氨酸和可溶性糖,提高SOD和POD的活性,以减缓盐胁迫对植株的伤害且具有一定的耐盐性。To explore the physiological response of Malus sieversii to salinity stress,Malus sieversii seedlings were cheared with six salinity levels（ 0,0. 2%,0. 4%,0. 6%,0. 8% and 1. 0% Na Cl） for 4 hours and seven time levels（ 0,4,8,12,16,20 and 24 hours） in 0. 4% Na Cl. Contents of proline,MDA,chlorophyll,and soluble sugar in leaves and activity of SOD and POD were mensurated. The results showed that proline content got the peak when Na Cl concentration was 0. 6% or 12 h in 0. 4% Na Cl,increasing 63. 1% and 27. 0% compared to control respectively. Increasing salt concentration and time resulted in higher levels for malondialdehyde content. Chlorophyll content was decreased first,subseguently increased and decreased once more. Among all treatment they were lower than control. Soluble sugar content and the activity of SOD and POD were increased first,then decreased. The results indicated that Malus sieversii could reduce the damage to the plant by accumulating more proline and soluble sugar and increasing the activity of SOD and POD. It was adapted to salinity stress to some extent.

关 键 词：新疆野苹果 盐胁迫 抗氧化酶 生理响应 

分 类 号：S661.1[农业科学—果树学]