丝瓜提取物对Aβ_(25-35)诱导的PC12细胞损伤的保护性研究  

The Protective Effect of Luffa Extract on Injury of PC12 Cells Induced by Aβ_(25-35)

作　　者：刘春杰[1] 董立珉[1] 郑亚萍[1] 康红钰[1] 

机构地区：[1]漯河医学高等专科学校,河南漯河462000

出　　处：《食品研究与开发》2016年第1期23-25,共3页Food Research and Development

基　　金：河南省骨干教师资助课题(2011GGJS-281);漯河医学高等专科学校校级科研课题(2014-S-LMC04)

摘　　要：观察丝瓜提取物（Luffa extract,LE）对β-淀粉样蛋白（Aβ25-35）诱导的PC12细胞损伤的保护作用。应用Aβ25-35造成神经细胞损伤模型,用四甲基偶氮唑盐微量酶反应（MTT）比色法检测各剂量组丝瓜提取物对PC12细胞活力的变化,检测过氧化氢酶（CAT）、谷胱甘肽过氧化氢酶（GSH-Px）活性水平,通过蛋白印迹试验（Western Blotting）来检测B细胞淋巴瘤基因-2（Bcl-2）和Bcl-2相关X蛋白（Bax）的表达水平。丝瓜提取物剂量组PC12细胞存活率高于模型组,GSHPx活性显著增强,CAT含量显著增加（P〈0.01或P〈0.05）,Bax表达水平降低,Bcl-2表达增高,与模型组比较,差异显著（P〈0.05～P〈0.01）。丝瓜提取物对Aβ25-35诱导的PC12细胞损伤有明显的保护作用。To study the protective effect of luffa extract on injury of PC12 cells induced by amyloid β protein（Aβ25-35）. Neurons injury model was induced by Aβ25-35. MTT assay was used to detect the cellular viability after different concentrations of luffa extract. The activity of catalase（CAT） and glutathione peroxidase（GSH-Px）were detected. The expression of Bcl-2 and Bax were detected by Western Blotting. Compared with the control group, the cellular viability of luffa extract group was increased, the activity of GSH-Px and CAT were significantly increased（P〈0.01, P〈0.05）, the expression of Bax was decreased and Bcl-2 was increased（P〈0.05-P〈0.01）. luffa extract can protect PC12 cells from Aβ（25-35 ）induced cytotoxicity.

关 键 词：丝瓜提取物 PC12细胞 MTT检测 CAT GSH-PX Bcl-2 Bax 

分 类 号：R285[医药卫生—中药学]