miR-19b-1在肾癌中的表达及临床意义的研究  

Expression and clinical significance of miR-19b-1 in renal cell cancer

作　　者：胡佳[1,2] 金露[2] 何韬[1,2] 史本涛[2] 来永庆[2] 倪梁朝[2] 

机构地区：[1]广州医科大学,广东广州510182 [2]北京大学深圳医院泌尿外科,广东深圳518036

出　　处：《现代泌尿外科杂志》2016年第3期222-225,共4页Journal of Modern Urology

基　　金：国家自然科学基金(No.81101922);广东省自然科学基金(No.S2012010008365)

摘　　要：目的检测肾癌细胞(ACHN、786O、769P)与人胚肾细胞(HEK-293T)、肾癌及对应癌旁组织的miR-19b-1的表达水平,并分析其与临床病理特征之间的关系。方法利用qPCR技术检测miR-19b-1在肾癌细胞与人胚肾细胞、肾癌组织与配对组织中的表达量,运用统计学方法分析其与临床病理之间有无相关性。结果 miR-19b-1在肾癌细胞中的表达量比正常细胞高,在肾癌组织中的表达量是对应癌旁组织的2.2倍,且其差异具有统计学意义(P<0.05),与患者的年龄、性别及病理分期无关(P>0.05)。结论肾癌组织及肾癌细胞中miR-19b-1表达上调,提示肾癌的发生可能与miR-19b-1上调有关。Objective To detect the expression of miR-19b-1in renal cell carcinoma（RCC）,paired tissues,kidney cancer cells（ACHN/786O/769P）and human embryo kidney cell line 293T（HEK-293T）,and to analyze its relation with clinicopathological features.Methods The expression level of miR-19b-1in renal carcinoma cells,normal kidney cells,renal tumor and corresponding adjacent tissues was detected with quantitative real-time PCR（qRT-PCR）,and the correlation with clinicopathological features was analyzed.Results The expression of miR-19b-1 in renal tumor cells was significantly higher than in normal renal cells.In RCC tissues,it was approximately2.2times than in paired healthy tissues.The difference was statistically significant（P〈0.05）.It was not related with the patient’s age,gender and pathological stage（P〉0.05）.ConclusionThe miR-19b-1 was up-regulated in renal cancer tissues and kidney tumor cells,which indicated that it might be related with the occurrence of renal cell carcinoma.

关 键 词：肾癌 MICRORNA microRNA-19b-1 表达 病理特征 

分 类 号：R737.11[医药卫生—肿瘤]