ggpS基因过表达对集胞藻PCC 6803甘油葡萄糖苷和甘油合成的影响  

Effects of ggpS over-expression on glycosylglycerol and glycerol biosynthesis of Synechocystis sp.PCC 6803

作　　者：马培珍[1,2] 谈晓明[2] 吕雪峰[2] 田继远[1] 

机构地区：[1]青岛农业大学食品科学与工程学院,山东青岛266109 [2]中国科学院青岛生物能源与过程研究所中国科学院生物燃料重点实验室,山东青岛266101

出　　处：《生物工程学报》2016年第3期347-354,共8页Chinese Journal of Biotechnology

基　　金：山东省科技发展计划(No.2014GSF121033);国家海洋局"全球变化与海气相互作用"专项(No.GASI-03-01-02-05)资助~~

摘　　要：为了研究甘油葡萄糖苷磷酸合成酶(GgpS)在集胞藻PCC 803甘油葡萄糖苷和甘油合成中的作用,本研究在前期获得高产甘油葡萄糖苷藻株的基础上分别过量表达来自于集胞藻PCC 6803自身和聚球藻PCC7002的甘油葡萄糖苷磷酸合成酶基因ggpS,并测定了在不同浓度NaCl胁迫时突变藻株的甘油葡萄糖苷和甘油积累量。结果发现获得的突变株甘油葡萄糖苷合成没有提高,但是甘油合成显著增强。此外,当培养基NaCl浓度从600 mmol/L提高到900 mmol/L时,集胞藻PCC 6803自身ggpS过表达藻株的甘油合成进一步提高75%。这些结果显示了GgpS在将碳代谢流导入集胞藻甘油合成途径中的作用。研究成果也为进一步通过基因工程改造提高集胞藻甘油葡萄糖苷和甘油合成效率奠定了基础。To study the roles of glucosylglycerol phosphate synthase（Ggps） in glucosylglycerol（GG） and glycerol biosynthesis, we over-expressed Ggps from either Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002 in a Synechocystis strain with a high GG titer, and determined the GG and glycerol accumulation in the resultant mutants grown under different NaCl-stress conditions. Ion chromatography results revealed that GG yield was not improved, but glycerol production was significantly enhanced by over-expression of Ggps from Synechocystis sp. PCC 6803（6803ggpS）. In addition, increasing the NaCl concentration of medium from 600 to 900 mmol/L led to a further 75% increase of glycerol accumulation in the mutant strain with 6803 ggpS over-expression. These findings show the role of ggpS in driving the carbon flux to the glycerol biosynthesis pathway, and will be helpful for further improvement of GG and glycerol production in Synechocystis.

关 键 词：甘油葡萄糖苷 甘油 集胞藻PCC6803 ggpS基因 基因工程 

分 类 号：Q943.2[生物学—植物学]