过氧乙酸离析法观测蓝靛果忍冬叶片脉序的方法研究  

Observation on the Characteristics of Lonicera caerulea Leaf by Segregation Method of Peroxyacetic Acid

作　　者：徐清华[1] 吴旭飞[1] БРЫКСИН Д.М. 张雪霞[1] 李富恒[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]俄罗斯米丘林园艺研究所,坦波夫州米丘林斯克市393774

出　　处：《黑龙江农业科学》2016年第3期80-83,105,共5页Heilongjiang Agricultural Sciences

基　　金：黑龙江省教育厅资助项目(12531013)

摘　　要：过氧乙酸离析法主要用于植物叶片表皮的观测,也可用于叶片脉序的观测,操作简单实用。为获得适于蓝靛果忍冬叶片脉序观测的最佳组合,以蓝靛果忍冬叶片为材料,采用单因素试验和正交试验对过氧乙酸浓度、水浴温度、水浴时间的处理条件进行了系统的研究。结果表明:在70℃水浴中用12%的过氧乙酸处理蓝靛果忍冬叶片3.5h或用14%的过氧乙酸溶液在60℃水浴中处理3.5h,得到的装片脉序观测效果最佳。Segregation method of Peroxyacetic acid was used to observe the epidermis of plant leaves and used for observing leaf nervation,it was a simple and practica method.Taking Lonicera caerulea L.var.edulis Turcz.ex Herd.f.edulis as material,single factor experiment and orthogonal experiment were designed for the treatment conditions of Peracetic acid concentration,water-bath temperature and water-bath time.Observing leaf nervation was used in order to obtain the optimal reaction condition.The results of orthogonal test showed that the best condition of observation effect was 12% peroxyacetic acid treatment by 3.5hwater bath（70℃）or14% peroxyacetic acid treatment by 3.5hwater bath（60℃）for fresh leaves.

关 键 词：蓝靛果忍冬 叶片 过氧乙酸离析法 脉序 正交试验 

分 类 号：S663.9[农业科学—果树学]