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作 者:郑吉顺 陈萌萌[2] 周翔天[2] 刘艳艳[2] 李家斌[2]
机构地区:[1]安徽医科大学第三附属医院消化内科与感染科,合肥230061 [2]安徽医科大学第一附属医院感染性疾病科,合肥230022
出 处:《中国临床药理学杂志》2016年第6期495-498,共4页The Chinese Journal of Clinical Pharmacology
基 金:国家自然科学基金资助项目(81172737)
摘 要:目的探讨白细胞介素-17(IL-17)A^F(IL-17A、IL-17B、IL-17C、IL-17D、IL-17E、IL-17F)在慢性乙型病毒性肝炎(乙肝)及肝硬化中的表达情况及临床意义。方法收集66例慢性乙肝患者(慢性乙肝组)和50例乙肝肝硬化(肝硬化组)患者的血清及粪便,以及30例健康志愿者(对照组)的血清。用荧光定量PCR检测乙肝病毒(HBV)DNA,用酶联免疫吸附试验法(ELISA)检测IL-17A^F、IL-1β、IL-6、趋化因子-13。比较3组IL-17A^F的表达情况及与细胞因子、肝功能、HBV DNA的相关性。结果慢性乙肝组及肝硬化组的IL-17A^F均明显高于对照组(P<0.01),但慢性乙肝组与肝硬化组间差异无统计学意义(P>0.05)。慢性乙肝组IL-17A^F与IL-1β、IL-6、CXCL-13呈正相关,与总胆红素呈负相关;IL-17C与总胆汁酸呈正相关;IL-17B,IL-17D,IL-17E与粪便HBV DNA呈正相关。肝硬化组IL-17B与IL-1β呈正相关;IL-17C与谷草转氨酶、碱性磷酸酶呈正相关,IL-17D与碱性磷酸酶呈正相关;IL-17A^F与血清及粪便HBV DNA均无相关性(P>0.05)。结论慢性乙肝及肝硬化患者均存在IL-17A^F高表达,但临床意义不完全相同。Objective To investigate the expression and compare the significance of interleukin-17( IL-17) A-F in patients with chronic hepatitis B( CHB) and hepatitis B virus( HBV) cirrhosis. Methods The stools and serum samples of 66 patients with CHB and 50 patients with HBV cirrhosis were collected,and the serum samples of 30 healthy volunteers were collected. HBV DNA in stool and serum were detected by real-time quantitative polymerase chain reaction( PCR).IL-17A-F,IL-1β,IL-6 and CXC chemokine ligand 13( CXCL-13)were detected by ELISA. Liver function was detected by automatic biochemical analyzer. Results The expression of serum IL-17A-F in patients with CHB or HBV cirrhosis were significantly higher than those in healthy volunteers( P〈0. 01),but no difference between patients with CHB and cirrhosis( P〈0. 05). In patients with CHB,IL-17A-F were positively correlated with IL-1β,IL-6 and CXCL-13,and negatively correlated with total bilirubin. IL-17 C was positively correlated with total bile acid. IL-17 B,IL-17 D and IL-17 E were positivelycorrelated with stool HBV DNA. In patients with HBV cirrhosis,IL-17 B was positively correlated with IL-1β.IL-17 C was positively correlated with aspartate aminotransferase and alkaline phosphatase. IL-17 D was positively correlated with alkaline phosphatase. IL-17A-F had no correlation with serum HBV DNA and stool HBV DNA( P〈0. 05). Conclusion There were high expressions of serum IL-17A-F in patients with CHB and HBV cirrhosis,but the clinical significance of IL-17A-F was different.
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