山茱萸果核提取物对佐剂性关节炎大鼠免疫调节因子的影响  

Effects of fructus corni officinalis fruit core extract on immune cytokine of adjuvant arthritis rats

作　　者：齐静姣[1] 张平[2] 郭进武[3] 路西明[4] 

机构地区：[1]河南科技大学医学院免疫教研室,河南洛阳471003 [2]河南科技大学第三附属医院内科,河南洛阳471003 [3]河南科技大学化工与制药学院公共化学教研室,河南洛阳471003 [4]河南科技大学医学院药理教研室,河南洛阳471003

出　　处：《中国临床药理学杂志》2016年第6期549-552,共4页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨山茱萸果核提取物(FCOFCE)对佐剂性关节炎大鼠免疫调节因子的影响及其抗炎机制。方法取40只Wistar大鼠构建佐剂性关节炎模型,并随机分为模型组、雷公藤组、低、中、高剂量FCOFCE组,每组8只;另取8只健康Wistar大鼠作为正常组。正常组、模型组均予以灌胃0.9%氯化钠,qd;雷公藤组予以灌胃0.01 mg·kg^(-1)雷公藤多苷,qd;低、中、高剂量FCOFCE组分别灌胃予以200,400,600 mg·kg^(-1)FCOFCE,qd。6组均连续给药4周。用跖围法测量大鼠足趾肿胀程度。用逆转录-聚合酶链反应检测大鼠血淋巴细胞白细胞介素-23(IL-23)、IL-17的mRNA的表达。用酶联免疫吸附法检测大鼠血清IL-23、IL-17的水平。结果与模型组比较,中、高剂量FCOFCE组大鼠的足趾肿胀度、血清IL-17和IL-23的含量、淋巴细胞IL-17和IL-23的mRNA表达水平均显著降低(P<0.05),而低剂量FCOFCE组上述指标降低不明显(P>0.05)。结论山茱萸果核提取物可抑制佐剂性关节炎大鼠的炎症反应,与降低IL-23和IL-17的表达水平有关。Objective To explore the effects of fructus corni officinalis fruit core extract（ FCOFCE） on immune cytokine of adjuvant arthritis rats,and to study the underlying anti-inflammatory mechanisms.Methods Adjuvant arthritis rat model was established with forty Wistar rats,after that,they were randomly divided into model group,tripterygium wilfordii polyglycoside（ TWP） group,low-dose,medium-dose and high-dose of FCOFCE group,8 rats in each group. Eight healthy Wistar rats was used as the normal control group. The normal group and model group were given the injection of 0. 9% Na Cl. TWP group was given the 0. 01 mg·kg^（-1）TWP. Low-dose,medium-dose and high-dose of FCOFCE group were respectively given 200, 400 and 600mg·kg^（-1）FCOFCE. Six groups were given the corresponding dose by intragastric administration once a day,for 4 weeks. The swollen volume of rats＇ rear claw were measured by plantar circumference. The interleukin（ IL）-23 mRNA and IL-17 mRNA expression in the blood lymphocytes of adjuvant arthritis rats were detected by reversing transcription-polymerase chain reaction. Serum IL-23 and IL-17 were measured in all the sera with sandwich ELISA method. Results Compared with modelgroup,medium-dose and high-dose of FCOFCE group had remarkable decreasions in the terms of swollen volume of the rats＇ rear claw,serum IL-17 and IL-23 contents and IL-17 mRNA and IL-23 mRNA expression levels（ P〈0. 05）. No obvious differences were found between low-dose FCOFCE group and model group in the aforementioned indexes（ P〈0. 05）. Conclusion FCOFCE intervention can reduce the inflammatory reaction of adjuvant arthritis rats,the possible mechanism may be related to its down regulation of IL-17 and IL-23 levels.

关 键 词：山茱萸果核提取物 佐剂性关节炎 白细胞介素-17 白细胞介素-23 

分 类 号：R285.5[医药卫生—中药学]