高温对西花蓟马卵巢发育及卵黄蛋白含量的影响  

Effects of high temperature on ovary development and yolk protein content of Frankliniella occidentalis (Thysanoptera: Thripidae)

作　　者：马亚斌[1] 孙丽娟[1] 李洪刚[2] 王思芳[1] 张彬[1] 郑长英[1] 万方浩[1,3] 

机构地区：[1]青岛农业大学农学与植物保护学院,山东省植物病虫害综合防控重点实验室,山东青岛266109 [2]山东省农药检定所,济南250100 [3]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2016年第2期127-137,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31372003);青岛市自然基金项目(13-1-4-163-jch);青岛农业大学研究生创新计划项目(7601214003);“泰山学者”建设工程专项

摘　　要：【目的】为探明短时高温热激后西花蓟马Frankliniella occidentalis(Pergande)生殖力和卵巢发育的相互关系。【方法】选择1龄、2龄若虫,预蛹,蛹和1日龄成虫,采用不同高温(41和45℃)短时(2 h)处理,观察卵巢发育及卵黄蛋白相对含量的变化。【结果】高温热激主要抑制了西花蓟马生殖器官和胚胎的正常发育,导致卵巢管数量减少,卵巢管和胚胎发育畸形;电泳结合不同的染色方法证明西花蓟马的卵黄蛋白为一种磷酸化、糖基化的脂蛋白,由3个亚基组成,分子量分别为116,70和47 k Da。除热激蛹期外,热激其他龄期的若虫对其当代成虫卵黄蛋白合成都起到抑制作用,且45℃热激1龄或2龄若虫后,其对卵黄蛋白相对含量的影响持续到了F_1代。【结论】温度对雌虫生殖力的影响是由于其对生殖器官发育和卵黄蛋白合成的影响引起的。本研究为西花蓟马的卵黄蛋白和卵黄发生的进一步研究奠定了基础。【Aim】This study aims to clarify the relationship between fecundity and ovary development of Frankliniella occidentalis（ Pergande）（ Thysanoptera： Thripidae）. 【Methods】The 1st instar nymphs,2nd instar nymphs,prepupae,pupae,and the 1-day-old adults were subjected to 41℃ and 45℃ heat shock,respectively,for 2 h and the changes in their ovary development and relative contents of yolk protein were observed. 【Results】The normal development of reproductive organs and embryos of F.occidentalis were inhibited by heat exposure,which resulted in the reduction in the number of ovarian tubes and deformity of ovarian tubes and embryo. The yolk protein of F. occidentalis was identified as a phosphorylated and glycosylated lipoprotein,consisting of three subunits with the molecular weight of116,70 and 47 k Da,respectively,through electrophoresis and different dyeing methods. The yolk protein synthesis of F_0 generation adults was inhibited when nymphs and prepupae were exposed to high temperature,and the effect of high temperature on the relative content of yolk protein continued to F_1 generation when the 1st or 2nd instar nymphs were exposed to high temperature（ 45℃）. 【Conclusion 】The effect of temperature on female fecundity of F. occidentalis is due to its influence on the development of reproductive organs and the synthesis of yolk protein. This study lays a foundation for the further research on yolk protein and vitellogenesis of F. occidentalis.

关 键 词：西花蓟马 热激 卵巢 卵黄蛋白 生殖力 

分 类 号：Q965[生物学—昆虫学]