有机溶剂致慢性毒性脑病中吸烟及代谢酶多态性的作用  

The role of smoking and metabolic enzyme polymorphisms in the organic solvent induced chronic encephalopathy

作　　者：王宏宇[1] 马玉美 崔建忠[1] 赵迪[3] 刘爱军[1] 洪军[1] 卢永刚[1] 王凯杰[1] 

机构地区：[1]唐山市工人医院神经外科,063000 [2]武警唐山市支队卫生队 [3]河北医科大学第一医院神经外科

出　　处：《中华劳动卫生职业病杂志》2016年第3期199-202,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

摘　　要：目的观察谷胱甘肽转移酶M1和T1（GSTM1，GSTFl）缺失突变基因型以及2种微粒体环氧化物水解酶（mEPHX）遗传多态性和吸烟对有机溶剂致慢性中毒性脑病（CTE）的影响。方法研究对象为115名有长期有机溶剂暴露史的患者，根据临床诊断患者分为CTE组（83例），无CET组（32例）。从患者白细胞中分离DNA用多重-聚合酶链式反应确定GSTM1和GSTT1缺失基因型。通过限制性片段长度多态性（PCR-RFLP）分析mEPHX的2种多态性。结果GSTMl缺失基因型对CTE相对风险明显提高（RR=2．35，95％CI：0.96～2．90）。根据患者的吸烟情况和基因型进行分类后，发现在吸烟患者中GSTM1缺失基因型患者CTE风险明显高于GSTM1（＋）基因型患者（RR=3．13，95％（211．2～3．7），2种mEPHX多态性均与CTE风险提高无关。结论GSTM1缺失基因型在个体对有机溶剂诱导的CTE易感性中有重要作用，同时受吸烟的影响。Objective The organic solvents and other exogenous compounds of metabolic enzymes genetic variation may affect the risk of the toxic effect of organic solvents exposure. Therefore, this research we observed the glutathione transferase M1 and T1 （GSTM1, GSTrl） deletion mutation genotype, two kinds of microsomal epoxide hydrolase （mEPHX） genetic polymorphism , organic solvents exposure and smoking effection in chronic cases of toxic encephalopathy（CTE） correlation. Methods The object was 115 patients who had a long history of organic solvents exposure, were divieded into two groups：CTE （n=83）, no CET （n=32） according to clinical diagnosis. DNA was isolated from patients in white blood cells through the muhiple- polymerase chain reaction to determine the loss of GSTM 1 and GST＇F1 genotype, two kinds of mEPHX polymorphism were analysised through the PCR- RFLP （restriction fragment length polymorphism）.Results The relative risk has obviously improved when lack of GSTM1 genotypes to CTE （RR=2.35, 95% CI 2.35 0.96）. in according to the patient＇s Smoking condition and classify genotype, patients lack of GSTM1 genotypes had a significantly higher risk CTE than GSTM 1 ＋genotype patients （RR =3.13, 95% CI 3.13 1.2）, both mEPHX polymorphisms had nothing to do with an increased risk of CTE. Conclusion The GSTM1 genotypes played an important role in the organic solvent induced the CTE of susceotibilitv.it was Influenced by the interaction between smokinz at the same time.

关 键 词：有机化学品 脑损害 慢性 多态性 单核苷酸基因 

分 类 号：R135.1[医药卫生—劳动卫生] R747.9[医药卫生—公共卫生与预防医学]