宁夏地区VDR基因多态性和糖尿病前期HOMA指数关系  

Association between VDR gene polymorphisms and HOMA index for prediabetes in Ningxia

作　　者：廖沙[1] 何俊[1] 李小霞[1] 许红霞[1] 刘秀英[1] 赵燚[1] 张毓洪[1] 

机构地区：[1]宁夏医科大学公共卫生学院,银川750004

出　　处：《卫生研究》2016年第2期184-188,共5页Journal of Hygiene Research

基　　金：国家自然科学基金(No.81160358)

摘　　要：目的探讨糖尿病前期维生素D受体(VDR)基因Bsm I和Fok I位点多态性对HOMA指数的影响。方法在宁夏地区2008—2012年开展的横断面研究的基础上,根据美国糖尿病学会(ADA)的糖尿病诊断标准将339例正常组和468例糖尿病前期组进行比较研究,收集人体测量数据,采集空腹肘静脉血进行血液生化检测和胰岛素检测,并提取全血DNA进行基因分型。结果糖尿病前期组的BMI、腰围、空腹血糖(FPG)、胰岛素抵抗指数(HOMA-IR)均高于正常组(P<0.05),而B细胞功能指数(HOMA-B)和胰岛素敏感度(HOMA-S)值低于正常组(P<0.05);Bsm I位点中,糖尿病前期组携带基因型BB/Bb个体的HOMA-B低于bb携带者(P<0.05),而HOMA-S值明显高于bb携带者(P<0.05);在控制变量后,低HOMA-B值对Bsm I位点中携带基因型BB/Bb和Fok I位点FF/Ff/ff基因型个体均为糖尿病前期的危险因素(OR>1,P<0.05),无基因型特异性,但基因型ff个体的危险度最高(OR=10.59),低HOMA-S值和低HOMA-IR值在携带基因型Ff的个体中,均为糖尿病前期的危险因素(OR>1,P<0.05)。结论在糖尿病前期,VDR基因多态性与HOMA指数存在一定关联,Bsm I位点多态性可影响HOMA-B,而Fok I位点多态性可不同程度影响HOMA-B、HOMA-S和HOMA-IR。Objective To explore the association between the vitamin D receptor（ VDR） gene polymorphisms and HOMA index in prediabetes. Methods On the basis of a cross-sectional study which was conducted in Ningxia during 2008—2012,339 controls and 468 subjects with prediabetes were selected according to ADA diabetes diagnosis standards. Anthropometric data and blood samples were collected in the field investigation. Blood biochemistry analyses and insulin determination were carried out in the laboratory. The whole blood DNA was extracted for genotyping. Results The BMI,WC,FPG and HOMA-IR of individuals with prediabetes were higher than those of the controls,while the HOMA-B and HOMA-S in cases were lower than those of the controls（ P〈0. 05）. In Bsm I,individuals with prediabetes carrying genotype BB /Bb showedlower HOMA-B than bb carrier,and they showed significantly higher HOMA-S than bb carriers（ P〈0. 05）. After adjusting age,sex,BMI,TC,TG and SBP,low level HOMAB index was the risk factor of prediabetes in individuals who carried genotype BB/Bb for Bsm I and genotype FF/Ff/ff for Fok I（ OR〉 1,P〈0. 05）,and the genotype ff got the highest risk level（ OR = 10. 59）. In Fok I,the Ff carriers with low level HOMA-S and HOMA-IR were also the risk factors of prediabetes（ OR 〉1,P〈0. 05）. Conclusion The VDR gene polymorphisms appeared to be associated with HOMA index in prediabetes. The Bsm I polymorphism seemed to influence HOMA-B,while the Fok I polymorphism influence HOMA-B,HOMA-S and HOMA-IR at different levels.

关 键 词：糖尿病前期 HOMA指数 维生素D受体基因 

分 类 号：Q587.1[生物学—生物化学] Q578