植物源正辛酸的抑藻效应及其机制  

Algae-inhibitory effect and mechanism of plant source n-caprylic acid

作　　者：陈永玲[1] 彭桂莹 韩玉珍[1] 张庭廷[1] 

机构地区：[1]安徽师范大学生命科学学院,生物环境与生态安全安徽省高校重点实验室,安徽省皖江城市带退化生态系统的恢复与重建协同创新中心,芜湖241000

出　　处：《卫生研究》2016年第2期189-193,199,共6页Journal of Hygiene Research

基　　金：国家自然科学基金(No.31170443)

摘　　要：目的研究从椰子油中提取的植物源正辛酸对铜绿微囊藻(Microcystis aeruginosa)的抑制效应及其机制。方法分别采用0、12.5、25、50和100μL/L的椰油正辛酸对铜绿微囊藻进行胁迫处理,测定其藻密度变化、细胞通透性改变以及藻细胞抗氧化能力的变化。结果随着椰油正辛酸浓度的增加,铜绿微囊藻生长受抑现象越明显。当椰油正辛酸浓度为100μL/L,处理时间为96 h时,其对铜绿微囊藻的抑制率达到99%。与对照组相比,实验组藻液的电导率、核酸含量、蛋白质含量明显升高;椰油浓度低于25μL/L时,SOD活性随椰油正辛酸浓度的增加而增强,但超过25μL/L时,其活性明显减弱。MDA含量始终随着椰油正辛酸浓度的增加而增加。结论椰油正辛酸对铜绿微囊藻具有明显的抑制作用,主要与其改变了细胞膜通透性、降低了抗氧化能力有关。Objective To research the algae-inhibitory effect and mechanism of a plant source n-caprylic acid extracted from coconut oil on M. aeruginosa. Methods The M. aeruginosa were treated with 0,12. 5,25,50 and 100 μL/L densities of coconut oil n-caprylic acid respectively. And the change of algal density, cell permeability and antioxidant ability were measured. Results The greater the concentration of coconut oil n-caprylic acid,the stronger the inhibition on M. aeruginosa. When the concentration was100 μL/L and processed time was 96 h,the inhibition ratio on M. aeruginosa reached99%. Compared with the control group, the algal liquid conductivity, nucleic acid content and protein content of experimental groups increased significantly. The SOD activity enhanced with the increasing of coconut oil n-caprylic acid concentration（ under25 μL/L）,but when beyond a certain concentration（ exceed 25 μL/L）,its activity weakened greatly. And the MDA content increased with the increasing of coconut oil ncaprylic acid concentration. Conclusion Coconut oil n-caprylic acid has a significantinhibiting effect on M. aeruginosa. Its mechanism may be largely related to cell membrane permeability change and antioxidant capacity reducing.

关 键 词：椰油正辛酸 铜绿微囊藻 抑制效应 

分 类 号：X524[环境科学与工程—环境工程] Q946.819[生物学—植物学]