DNA流式细胞术在植物遗传及育种中的应用  

Application of DNA Flow Cytometry to Plant Genetics and Breeding

作　　者：金亮[1] 徐伟韦[2] 李小白[3] 刘建新[1] 田丹青[1] 葛亚英[1] 潘晓韵[1] 王炜勇[1] 

机构地区：[1]浙江省农业科学院花卉研究开发中心,浙江省萧山棉麻研究所,杭州311202 [2]慈溪市农业技术推广中心,慈溪315300 [3]浙江省农业科学院园艺研究所,杭州310021

出　　处：《中国细胞生物学学报》2016年第2期225-234,共10页Chinese Journal of Cell Biology

基　　金：浙江省农业科学院科技创新能力提升工程协同创新项目(批准号:2014CX014);浙江省农业科学院青年科技人才培养项目(批准号:2015R25R08E04);杭州市种子种苗专项(批准号:20140932H19)资助的课题~~

摘　　要：DNA流式细胞术是一种分析和测定分离细胞核DNA含量的方法。该方法的关键技术包括制备完整细胞核悬浮液、核酸荧光染料染色、根据相对荧光密度(DNA含量)对细胞核进行分类。由于样品制备及分析方便快捷,该技术已成为植物倍性鉴定、基因组大小测定、生殖途径鉴定等研究的重要工具。该文在对DNA流式细胞术基本原理简要介绍的基础上,对其在植物遗传和育种中的应用进行了综述。DNA flow cytometry is a method for analysis and estimation of DNA content of isolated cell nuclei. The keys to this method include preparation of aqueous suspensions of intact nuclei, staining the nuclei with a nucleic acid fluorochrome, and classification of the stained nuclei based on their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA flow cytometry has become an important tool for plant ploidy screening, estimation of genome size and determination of reproductive pathway and so on. In this review we gave a brief introduction of the basic principles of DNA flow cytometry, and reviewed its applications in plant genetics and breeding.

关 键 词：流式细胞术 植物 基因组大小 细胞核DNA含量 遗传 育种 

分 类 号：Q943[生物学—植物学]