原发性胃癌患者舌苔变化与转化生长因子α基因单核苷酸多态性位点分布的相关性  

Correlation between Changes of Coated Tongue and Transforming Growth Factor-α Gene Single Nucleotide Polymorphic Site Distribution in Patients with Primary Gastric Cancer

作　　者：王瑞[1] 张军峰[1] 吴娟[1] 杨学文[2] 王瑞平[2] 邱红[3] 詹瑧[1] 杨亚平[1] 

机构地区：[1]南京中医药大学基础医学院,江苏省南京市210023 [2]江苏省中医院 [3]中国人民解放军第八一医院

出　　处：《中医杂志》2016年第7期596-600,共5页Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81473593,81473458)

摘　　要：目的探讨原发性胃癌患者舌苔形成的分子遗传学机制。方法以原发性胃癌为基础病种,基于387例原发性胃癌患者和392例正常对照人群进行临床对照研究,记录受试者苔色和苔质,苔色包括白苔、黄苔和其他,苔质包括薄苔、厚苔、剥苔。利用连接酶检测反应检测受试者外周静脉血转化生长因子-α(TGF-α)基因8个单核苷酸多态性(SNP)位点分布情况,分析苔色、苔质与TGF-α基因SNP位点的相关性。结果仅携带SNP rs11466285 TC/TT基因型的原发性胃癌患者和正常对照人群苔色分布差异无统计学意义(P=0.122),其他各基因型苔色和苔质在两组受试者的分布差异均有统计学意义(P<0.01)。SNP rs503314 GG基因型、rs3732253 TC/TT基因型和rs538118、rs2166975显著影响苔色变化(P<0.05),SNP rs503314 GC/CC基因型和rs538118显著影响苔质变化(P<0.05)。结论原发性胃癌患者舌苔形成可能与TGF-α基因多态性相关。Objective To study molecular genetics mechanism of tongue coating formation in patients with primary gastric cancer. Methods Regarded primary gastric cancer as basic disease. Based on 387 cases of primary gastric cancer patients and 392 cases of normal control subjects,the researchers recorded tongue coating colour and nature including white,yellow and others in color and thin,thick and exfoliated in nature. The researchers used ligase detection reaction to detect distribution of eight single nucleotide polymorphic（ SNP） site of transforming growth factor-α（ TGF-α） gene in peripheral venous blood to analyze correlation between coating colour and nature with SNP site of TGF-α gene. Results There was no statistically difference in distribution of coating color between normal control people and primary gastric cancer patients carried with only SNP rs11466285 TC / TT genotype（ P = 0. 122）,but there was statistically difference in distribution of coating colour and tongue nature between normal control people and those patients with other various genotypes（ P 〈 0. 01）. SNP rs503314 GG genotype,rs3732253 TC / TT genotype and rs538118,rs2166975 significantly influenced changes of tongue coating color（ P 〈 0. 05）,while SNP rs503314 GC /CC genotype and rs538118 significantly influenced changes of tongue nature（ P 〈 0. 05）. Conclusion The formation of tongue coating of primary gastric cancer patients may be associated with polymorphism of TGF-α gene.

关 键 词：原发性胃癌 舌苔 转化生长因子Α 单核苷酸 基因多态性 

分 类 号：R273[医药卫生—中西医结合]