丙酮法和热乙醇法测定浮游植物叶绿素a的方法比对  

Comparison of Two Methods for Measurement of Phytoplanktonic Chlorophyll-a

作　　者：陈明华[1,2,3] 谢良国 付志强[3] 邹俊宇 曾繁翔 

机构地区：[1]南昌大学鄱阳湖环境与资源利用教育部重点实验室,江西南昌330031 [2]南昌大学资源环境与化工学院,江西南昌330031 [3]南昌大学生命科学学院,江西南昌330031

出　　处：《环境监测管理与技术》2016年第2期46-48,共3页The Administration and Technique of Environmental Monitoring

基　　金：国家级大学生创新创业训练计划基金资助项目(201310403013);江西省教育厅科技计划基金资助项目(GJJ14149)

摘　　要：将丙酮法和热乙醇法用于测定浮游植物叶绿素a,分析水样中是否加入碳酸镁、水样体积、萃取时间、萃取剂、破碎方法、离心时间、水样存放时间和滤膜样品存放时间等8个因素对测定的影响,并确定最佳试验条件。结果表明:2种方法的测定值之间有较好的线性关系,相比丙酮法,热乙醇法更具优势。Chlorophyll-a was determined by acetone method and hot-ethanol method. Eight factors( whether the magnesium carbonate was added or not,sample volume,extraction time,extraction solvent,crushing method,centrifugal time,storage time of water sample and filter membrane) were analyzed to determine the best test condition. Experimental results showed that the measured values were good linear correlation between acetone method and hot-ethanol method. Hot-ethanol shows an advantage over acetone method.

关 键 词：叶绿素A 浮游植物 热乙醇法 丙酮法 分光光度法 水质 

分 类 号：O657.32[理学—分析化学]