高效液相色谱法测定咪唑立宾的血药浓度  

Determination of Mizoribine in Human Serum by HPLC

作　　者：赵宇蕾[1] 芮建中[1] 孙胜利[1] 曹晓梅[1] 周国华[1] 

机构地区：[1]南京军区南京总医院药理科,南京210002

出　　处：《药学与临床研究》2016年第2期125-127,共3页Pharmaceutical and Clinical Research

基　　金：南京军区南京总医院院管课题(2011058)

摘　　要：目的:建立人血清中咪唑立宾(MZR)的高效液相色谱测定方法,为临床MZR的个体化用药提供方法学参考。方法:采用10%高氯酸直接沉淀血清蛋白,色谱柱为Megres C_(18)(4.6 mm×250 mm,5μm),流动相为甲醇-40 mmol·L^(-1)磷酸盐缓冲液(p H=3.5,2∶98,v/v),流速为0.9 m L·min^(-1),检测波长为280 nm。结果:MZR在0.05~10μg·m L^(-1)范围内线性关系良好(r=0.9998),批内、批间准确度偏差小于4.6%,批内、批间精密度RSD<7.9%,在室温放置、冰冻、反复冻融条件下考察样品稳定性,MZR均保持稳定,偏差小于4.9%。结论:高效液相色谱法测定MZR血药浓度的方法快速、准确、灵敏度高,适合在治疗药物监测中常规应用。Objective: To es tablish an HPLC method for determining mizoribine in human serum for clinical individualized medicine. Methods: After precipitation of serum proteins with 10% perchloric acid,mizoribine was determined by HPLC on a reversed phase Megres C_(18)column(4.6 mm×250 mm, 5 μm). The mobile phase was a mixture of methanol and 40 mmol·L^(-1)phosphate buffer adjusted to p H 3.5, in the ratio of 2∶98 and delivered at a flow rate of 0.9 m L·min^(-1). The UV detection was set at 280 nm. Results: The peak area for mizoribine was linearly related to its concentrations, which ranged from 0.05 to 10 μg·m L^(-1)(r =0.9998). The intra- and inter-day relative standard deviation values were within 7.9%, the extraction recoveries were over 95.3%. The samples, unprocessed, processed, in store or after 3 cycles of freeze and thaw processes, were stable. Conclusions: The established HPLC method is fast, sensitive, and accurate. It could be applied to therapeutic drug monitoring.

关 键 词：咪唑立宾 高效液相色谱法 血清浓度 

分 类 号：R969.1[医药卫生—药理学]