HBV前S1抗原与e抗原联合检测的临床意义  

The clinical significance of the combinationl detection of HBV pre-S1Ag and HBeAg

作　　者：王春苗[1,2] 朱锦宏[1] 吴晓蔓[1] 

机构地区：[1]广州医科大学附属第二医院检验科,广东广州510260 [2]广东省人民医院检验科,广东省医学科学院,广东广州510080

出　　处：《热带医学杂志》2016年第2期186-188,共3页Journal of Tropical Medicine

基　　金：广州市医药卫生科技项目(20141A011082)

摘　　要：目的探讨HBV感染者血清pre-S1Ag、pre-S1Ag联合HBe Ag与HBV感染传统标志物之间的关系,以评价其在临床诊疗中的作用。方法采用ELISA法对373例HBV感染者pre-S1Ag及HBV血清标志物进行检测,采用FQ-PCR法对HBV DNA进行平行检测。结果 373例HBV感染者中,HBe Ag、pre-S1Ag、pre-S1Ag联合HBe Ag检测与HBV DNA的总符合率分别为54.4%、75.1%、85.8%;158例HBe Ag阳性血清中,HBV DNA与pre-S1 Ag的阳性率分别为87.3%、68.4%,差异有统计学意义(P<0.05);215例HBe Ag阴性血清中,HBV DNA与pre-S1Ag的阳性率分别为69.8%、61.9%,差异无统计学意义(P>0.05);pre-S1Ag联合HBe Ag阳性率随乙肝感染者病程进展呈上升趋势。结论 pre-S1Ag能很好地反映体内HBV复制情况,其阳性率与HBV DNA具有较高的一致性;且pre-S1Ag联合HBe Ag检测阳性率与乙肝感染者病程进展密切相关,能适时地指导临床根据患者病情针对性地治疗。Objective To study the combined detection of pre-S1 Ag and e Ag on HBV replication and its clinical significance. Methods pre-S1 Ag and HBV M were detected by enzyme-link immunosorbent assay（ELISA） while HBVDNA was measured by real-time fluorescence quantitative polymerase chain reaction（FQ-PCR） in 373 hepatitis B patients. Results The total coincidence rates of HBe Ag, pre-S1 Ag, pre-S1Ag＋HBe Ag and HBV DNA were 54.4%,75.1% and 85.8%, respectively in 373 hepatitis B virus infected patients. Positive rates of HBV DNA and pre-S1 Ag were 87.3%, and 68.4% in 158 HBe Ag positive samples, respectively, showing significant difference（P〈0.05）. While positive rates were 69.8%, 61.9% in 215 HBe Ag negative samples, showing no significant difference（P〈0.05）. The positive rate of pre-S1Ag＋HBe Ag increased with the progress of disease in hepatitis B patients. Conclusion The positive rate of pre-S1 Ag can reflect HBV replication in vivo situation, and is consistent with HBV DNA detection. The positive rate of pre-S1 Ag plus HBe Ag is closely related to disease progression and can provide clinical guidance for targeted treatment according to the condition of patients.

关 键 词：乙型肝炎病毒 PRE-S1抗原 HBE AG HBV DNA 

分 类 号：R392.11[医药卫生—免疫学]