威灵仙总皂苷对实验性非酒精性脂肪性肝炎大鼠氧化应激的干预作用  

Effect of total saponins of Radix clematidis on oxidative stress in a rat model of non-alcoholic steatohepatitis

作　　者：高璠[1] 琚坚[1] 王伟[2] 闫福媛 王晓青[1] 

机构地区：[1]昆明医科大学第二附属医院特需病房科,云南省昆明市650101 [2]昆明医科大学第二附属医院急诊医学部,云南省昆明市650101

出　　处：《世界华人消化杂志》2016年第7期1070-1075,共6页World Chinese Journal of Digestology

摘　　要：目的:观察威灵仙总皂苷干预实验性非酒精性脂肪性肝炎(non-alcoholic steatohepatitis,NASH)大鼠模型氧化应激的疗效,为临床应用威灵仙总皂苷防治NASH提供科学依据.方法:采用高脂饲料饲养SD大鼠建立实验性NASH大鼠模型,并给予威灵仙总皂苷灌胃干预,通过检测空白组、模型组、威灵仙总皂苷组和易善复组下腔静脉血清中总抗氧化能力(total antioxidant capacity,T-AOC)、丙二醛(malondialdehyde,MDA)的含量以及肝组织上清液中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、过氧化氢酶(catalase,CAT)、超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)的含量,并分别统计分析各组间氧化能力的差异和抗氧化能力的差异,观察威灵仙总皂苷干预实验性NASH大鼠模型氧化应激的疗效.结果:威灵仙总皂苷组NASH大鼠与模型组相比,血清和肝组织MDA含量均明显降低(均P<0.01),血清T-AOC和肝组织GSH-Px、CAT、SOD含量均明显升高(均P<0.01);威灵仙总皂苷组NASH大鼠与易善复组相比,血清MDA、T-AOC和肝组织MDA、GSH-Px、CAT、SOD含量均相当(均P>0.05).结论:威灵仙总皂苷可有效改善NASH大鼠的氧化应激,疗效与易善复相当.AIM：To observe the effect of total saponins of Radix clematidis on oxidative stress in a rat model of non-alcoholic steatohepatitis（NASH）,in order to provide a scientific basis for the prevention and treatment of NASH with total saponins of Radix clematidis in clinical practice.METHODS：A rat model of NASH was developed by feeding SD rats a high-fat diet.The rats were divided into a blank group,a model group,a total saponins of Radix clematidis group and a polyene phosphatidylcholine group.After intervention with total saponins of Radix clematidis,the contents of total antioxidant capacity（T-AOC）,malondialdehyde（MDA） in the serum from the inferior vena cava and the contents of glutathione peroxidase（GSH-Px）,catalase（CAT）,superoxide dismutase（SOD） and MDA in the supernatants of liver tissue were detected.The differences in oxidation capacity and antioxidant capacity were compared among all groups.RESULTS：Compared with the model group,NASH rats treated with total saponins of Radix clematidis had lower contents of MDA in serum and liver tissue（P 0.01 for both）,and higher contents of T-AOC in serum,as well as GSH-Px,CAT,and SOD in liver tissue（P 0.01 for all）.Compared with the polyene phosphatidylcholine group,NASH rats treated with total saponins of Radix clematidis had similar serum MDA and T-AOC levels as well as hepatic levels of MDA,GSH-Px,CAT and SOD（P 0.05 for all）.CONCLUSION：Total saponins of Radix clematidis improve oxidative stress in rats with experimental NASH,and their therapeutic effect is equivalent to that of polyene phosphatidylcholine.

关 键 词：威灵仙总皂苷 非酒精性脂肪性肝炎 氧化应激 

分 类 号：R285.5[医药卫生—中药学]