模拟微重力对猕猴肺组织趋化因子CCL20及其受体CCR6表达的影响  

Effect of simulated weightlessness on the expression of chemokine CCL20 and it's receptor CCR6 in lung of rhesus monkey

作　　者：许崇玉 王萍[1] 陈杨[2] 朱敏立[2] 司少艳[3] 蔡艺灵[4] 马华松[5] 陈志明[5] 

机构地区：[1]安徽医科大学解放军306临床学院,北京100101 [2]解放军第306医院呼吸与危重症学科,北京100101 [3]解放军第306医院特种医学实验研究中心,北京100101 [4]解放军第306医院神经内科,北京100101 [5]解放军第306医院骨科,北京100101

出　　处：《安徽医科大学学报》2016年第4期484-488,共5页Acta Universitatis Medicinalis Anhui

基　　金：总装备部试验技术研究重点项目(编号:SMFA13K02)

摘　　要：目的探讨模拟微重力对猕猴肺组织CC亚族趋化因子配体20(CCL20)及趋化因子受体6(CCR6)mRNA和蛋白表达的影响。方法 15只猕猴分为3组,每组5只:对照组、模拟组、恢复组。HE观察猕猴肺组织结构,实时荧光定量PCR、免疫组化法检测肺组织中CCL20及CCR6 mRNA和蛋白表达。结果模拟组和恢复组猕猴肺组织可见肺泡间隔增厚,肺间质内及支气管旁可见淋巴细胞浸润,恢复组较模拟组减轻。模拟组、恢复组CCL20 mRNA的表达水甲较对照组增高,但差异无统计学意义。模拟组CCR6 mRNA的表达水平较对照组、恢复组显著增高(P<0.01)。模拟组CCL20及CCR6蛋白表达较对照组、恢复组显著升高(p<0.05)。结论中、长期模拟微重力可引起肺组织结构破坏、淋巴细胞浸润,并可引起猕猴肺组织中CCL20及CCR6表达增强。Objective To investigate the effect of simulated weightlessness on the mRNA and protein expression of ehemokine CCL20 and it＇s receptor CCR6 in lung of rhesus macaque. Methods Fifteen healthy young male rhesus monkeys were randomly divided into 3 groups： control group, simulated group and recovery group. HE staining was used to observe the histopathological structure changes of pul-monary tissues. And the mRNA and protein expres- sion of CCR6 and CCL20 in lung tissue were detected by immunohistochemistry and quantitative real-time PCR（ Q- PCR）. Results Compared with the control group, histopathological examination revealed alveolar septal thicken- ing, and alveolar and interstitial lymphocytic infiltration in simulated group and recovery group, and the pathologi- cal changes in recovery group were lighter than those in simulated group. The expressions of CCL20 mRNA in simu- lated group and the recovery group were higher than that in the control group, but there was no significant differ- ence; the expression of CCR6 mRNA in simulated group was significantly higher than that in the control group and the recovery group（P 〈0. 01 ） , but there was no significant difference between the control group and the recovery group. Immunohistochemistry results showed that CCL20 and CCR6 were expressed in the lung tissues of each group, but the expression of CCL20 was weak. The positive cells were found mainly in the cytopl-asm of bronchial and alveolar epithelial cells and vascular endothelial cells. The protein expression of CCL20 and CCR6 in simulated group were significantly higher than that in the control group and the recovery group （P 〈 0. 05 ）. Conclusion Me- dium or long term simulated weightlessness can induce the destruction of lung tissue structure and infiltration of lymphocytes, and it can also significantly enhance the mRNA and protein expression of CCL20 and its receptor CCR6 in lung tissues.

关 键 词：模拟微重力 猕猴 肺组织 CCL20 CCR6 

分 类 号：R563.9[医药卫生—呼吸系统] V7[医药卫生—内科学]