呼吸道合胞病毒空斑形成实验的条件优化  

Condition optimization for plaque forming test of respiratory syncytial virus

作　　者：于莉[1] 吴璇[1] 汪旻旻 黄升海[1] 

机构地区：[1]安徽医科大学基础医学院微生物学教研室,合肥230032

出　　处：《安徽医科大学学报》2016年第4期595-598,共4页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81371797);安徽高校省级自然科学研究重点项目(编号:KJ2012A152)

摘　　要：采用空斑形成实验方法测定呼吸道合胞病毒（RSV）的滴度,加入覆盖层倒置培养27、30、34、40h后经固定染色、剔除覆盖层后,可见清晰透明空斑,边缘清晰、形态均为圆形或类圆形,空斑直径分别为1、1.5、3、5mm左右。加入覆盖层后培养病毒的时间可以影响RSV最终所形成的空斑大小,并且随着时间的延长空斑逐渐增大,在30～34h所形成的空斑大小最佳,易于计数。病毒的稀释度也会影响空斑最终形成的数量,且随着稀释度的增加,空斑数量逐渐减少。Respiratort syncytial virus（RSV） titers were investigated by plaque formation assay. Clarity and bound- arv clear plaques could be visible afler fixed dyeing and removed eovers after joining eovering cultivation 27, 30,34, and 40 h. Round or class round plaque morphologies and plaques in 1, 1.5, 3 and 5 mm diameter were formed in different layer inversion training time, respectively. Plaques size which was formed by RSV could be af- fected by virus incubation time after added layer and plaques dimensions were increased gradually with the time ex- tending in the plaque formation assay for RSV. The plaques were easy to be counted during 30 -34 h in which the best size and number of plaques could be detected. The number of plaques formed eventually could also be affected by the dilution of virus and with the increase of dilution degrees, the reduction of plaque quantities was observed.

关 键 词：呼吸道合胞病毒 空斑形成实验 甲醛-结晶紫 

分 类 号：R373.14[医药卫生—病原生物学]