LED光源对人视网膜色素上皮细胞分泌单核细胞趋化因子-1和白细胞介素-8的影响  

Effects of light emitting diode on expression of MCP-1 and IL-8 in cultured human retinal pigment epithelial cells

作　　者：彭素芬[1] 韩泉洪[1] 李丽华[1] 南莉[1] 

机构地区：[1]天津医科大学眼科临床学院天津医科大学天津市眼科医院天津市眼科学与视觉科学重点实验室天津市眼科研究所,天津市300020

出　　处：《眼科新进展》2016年第3期201-205,共5页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:61178081);天津市应用基础与前沿技术研究计划(重点项目)(编号:14JCZDJC36300)~~

摘　　要：目的观察LED光源对人视网膜色素上皮(retinal pigment epithelial cells,RPE)细胞增生及分泌单核细胞趋化因子-1(monocyte chemotactic protein-1,MCP-1)和白细胞介素-8(interleukin-8,IL-8)的影响。方法传代培养的ARPE细胞分别置于500 lux、1000 lux的LED白光、蓝光、绿光中分别照射6 h、12 h、24 h,并分别设置对照组避光培养。采用MTT法检测RPE细胞的增生值(A_(460)),分别使用Real-time PCR和ELISA法检测各组RPE细胞MCP-1和IL-8的表达水平。结果 MTT法检测细胞增生值显示:蓝光、白光各组比较差异均有统计学意义(均为P<0.05),白光500 lux 24 h和1000 lux 12 h、24 h,蓝光各照度6h、12 h、24 h,RPE细胞增生值均较对照组低,差异均有统计学意义(均为P<0.05),细胞增生值随着白光和蓝光光照强度及光照时间的增加逐渐下降。RT-PCR结果显示,各组RPE细胞中MCP-1和IL-8的mRNA比较差异均有统计学意义(均为P<0.05)。其中白光500 lux 24 h和1000 lux 24 h,蓝光500 lux 12 h、24 h和1000 lux 12 h、24 h,绿光500 lux 24 h和1000 lux 24 h,各组RPE细胞中MCP-1和IL-8的mRNA表达水平较对照组高,差异均有统计学意义(均为P<0.05)。ELISA结果显示,白光500 lux 24 h和1000 lux 12 h、24 h,蓝光500 lux 12 h、24 h和1000 lux 12 h、24 h,绿光500 lux 24 h、1000 lux 24 h,与对照组相比,RPE细胞MCP-1、IL-8分泌量增加,差异均有统计学意义(均为P<0.05);蓝光在500 lux 24 h和1000 lux 12 h、24 h,MCP-1和IL-8分泌量较白光、绿光增加,差异均有统计学意义(均为P<0.05)。结论 LED白光、蓝光和绿光能以照度和时间依赖性影响ARPE细胞增生及分泌MCP-1和IL-8,以蓝光更为显著。Objective To observe the effects of light emitting diode（LED） on the expression of monocyte chemotactic protein-1（MCP-1） and interleukin-8（IL-8） in cultured human retinal pigment epithelial（RPE） cells. Methods C ultured human RPE-19 cells were divided into LED white light,blue light,green light and control group. The LED illumination were 500 lux and 1000 lux for 6 hours,12 hours and 24 hours,respectively. While the control groups were cultured without light exposure. The growth and proliferation were assessed using MTTas absorbance（A_（460））. The relative expression of mRNA and protein of MCP-1 and IL-8 in the cells were assayed by real-time PC R and enzyme linked immunosorbent assay（ELISA）,respectively.Results The A_（460） values of the groups exposed to white light and blue light were significant different（all P 〈 0. 05）. Compared with the control group,the A_（460） values were decreased significantly after exposure to white light with 500 lux for 24 hours and 1000 lux for 12 hours,24 hours,blue light for 6 hours,12 hours and 24 hours（all P 〈 0. 05）,and had a tendency to decrease gradually along with illumination intensity and exposure time. RT-PCR revealed the considerable difference about expression of M C P-1 and IL-8 mRNA in RPE cells among these groups（all P 〈 0. 05）. Among these groups,the expression of M C P-1 and IL-8 mRNA after exposed to white light with 500 lux for 24 hours and 1000 lux for 24 hours,blue light with 500 lux for 12 hours,24 hours and 1000 lux for 12 hours,24 hours,and green light with 500 lux for 24 hours,1000 lux for 24 hours were increased significantly when compared with the control group（all P 〈 0. 05）. ELISA showed that compared with the control group,the concentrations of MCP-1 and IL-8 were increased significantly after exposed to white light with 500 lux for 24 hours,1000 lux for 12 hours,24 hours,blue light with 500 lux for 12 hours,24 hours,1000 lux for 12 hours,24 hours,and green light with 500 lux for 24 hours,1000 l

关 键 词：人视网膜色素上皮细胞 单核细胞趋化因子-1 白细胞介素-8 光损伤 

分 类 号：R544.1[医药卫生—心血管疾病]