激光光凝法诱导大鼠高眼压模型的有效性评估  

Efficiency evaluation on laser-induced hypertensive rat model

作　　者：刘丽芳[1] 黄楚开[1] 林洪杰[1] 张铭志[1] 

机构地区：[1]汕头大学.香港中文大学联合汕头国际眼科中心,广东省汕头市515041

出　　处：《眼科新进展》2016年第3期206-209,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30672282)~~

摘　　要：目的评估激光光凝法诱导大鼠高眼压模型的有效性。方法 532 nm激光光凝小梁网法诱导大鼠左眼高眼压(n=45,右眼为对照眼),评估激光后大鼠眼压、视网膜神经节细胞及视网膜电图的变化;采用Tono-Lab眼压计测量清醒状态下的双眼眼压,每天1次;激光后3周,采用双盲法计数双眼视网膜铺片上免疫组织化学法标记的阳性节细胞数;记录大鼠双眼基线及处死前的视网膜电图。结果激光后90.70%(39/43眼)的大鼠眼压升高,其中需要第2次激光者占74.42%(32/43眼)。激光后右眼的眼压为(16.13±1.11)mm Hg(n=39;1 k Pa=7.5 mm Hg);左眼中有28.21%眼(n=11)眼压升高(18.27±2.53)d,其余眼(n=28)眼压升高(7.93±2.40)d,两者的眼压分别为(35.59±4.57)mm Hg、(21.56±3.06)mm Hg,两者的峰值眼压分别为(63.36±4.23)mm Hg、(49.43±7.22)mm Hg;实验眼与对照眼之间的峰值眼压差为(31.13±8.64)mm Hg,累积眼压差为(182.31±140.35)mm Hg,左右眼的眼压相比差异有显著统计学意义(P=0.000)。激光后3周,两个高眼压组的节细胞数相比,差异无统计学意义(P=0.693),左眼的节细胞丢失率为67.4%±14.8%,与右眼相比差异有显著统计学意义(P=0.000)。左眼视网膜电图明适应b波、Ph NR波及暗适应b波的潜伏期与激光前相比,差异均有统计学意义(P=0.000、0.046、0.000),其振幅与激光前相比,差异也均有显著统计学意义(P=0.001、0.000、0.000),而明适应a波的潜伏期、振幅及暗适应a波的潜伏期与激光前相比,差异均无统计学意义(P=0.138、0.198、0.092)。结论激光光凝法诱导大鼠眼压升高成功率较高,但持续时间有限,峰值眼压过高,且需重复激光,高眼压引起的节细胞丢失率高,视网膜电图提示视网膜损伤主要累及双极细胞及节细胞。Objective To evaluate the efficiency of laser-induced hypertensive rat model.Methods Experimental glaucoma was induced in left eyes of 45 Fischer rats by a diode laser with wavelength of 532 nm aimed at trabecular meshwork. Efficiency of glaucomatous model was evaluated by assessing the success rate and duration of high intraocular pressure and IOP induced retinal histological and functional changes. IOP was measured by a Tonolab tonometer in conscious rats every day. At 3 weeks after laser,the degree of retinal ganglion cells（RGC） loss was assessed by a masked,semiautomated counting of Tuj-1 labeled R GC,electroretinography（ERG） was recorded from both eyes using custom made electrodes at baseline and just prior to euthanasia. Results After laser photocoagulation,the success rate of high IOP was 90. 7%（39 /43）,the rate for second laser was 74. 42%（32 /43）. IOP in right eyes（n = 39）,28. 21% of left eyes（n = 11） and 71. 79% of left eyes（n = 28） were（16. 13 ± 1. 11） mm Hg（1 k Pa = 7. 5 mmHg）,（35. 59 ± 4. 57） mm Hg and（21. 56 ± 3. 06） mm Hg,respectively,peak IOP in these eyes were（22. 36 ± 2. 88） mm Hg,（63. 36 ± 4. 23） mm Hg and（49. 43 ± 7. 22） mm Hg,respectively,peak IOP difference and cumulative IOP difference between experimental and control eyes were（31. 13 ± 8. 64） mm Hg and（182. 31 ± 140. 35） mm Hg,respectively,the difference between left and right eyes was statistically significant（P = 0. 000）. High IOP in left eyes persisted for（18. 27± 2. 53） days（n = 11） and（7. 93 ± 2. 40） days（n = 28）,respectively. At 3 weeks after laser photocoagulation,R GC loss in two left-eye groups was not statistically significant（P = 0. 693）,how-ever,in comparison with right eyes,R GC loss in left eyes was（67. 4 ± 14. 8） %（P = 0. 000）.There were significantly prolonged latencies for photopic b-wave,PhNR wave and scotopic bwave（P = 0. 000,P = 0. 046,P = 0. 000）,and the amplitude of these waves were also significantly decreas

关 键 词：青光眼模型 激光光凝法 视网膜电生理 鼠 免疫组织化学 

分 类 号：R988.1[医药卫生—药品]