虎杖饮片指纹图谱的研究及其耐用性考察  

The fingerprint and durability study on various regions of Polygonum cuspidatum

作　　者：蔺红伟[1] 朴淑娟[1] 江春霞[1] 李静娴[1] 陆文铨[1] 陈万生[1] 

机构地区：[1]第二军医大学附属长征医院药学部,上海200003

出　　处：《药学实践杂志》2016年第2期174-176,187,共4页Journal of Pharmaceutical Practice

摘　　要：目的为用数据识别虎杖饮片的真伪及评价其质量提供技术支持。方法采用高效液相色谱(HPLC)法分析虎杖饮片提取物的指纹图谱,色谱柱:Agilent ZORBAX SB-C18柱(4.6mm×250mm,5μm);流动相:0.1%甲酸水溶液-乙腈(A-B);梯度洗脱;检测波长306nm;流速1ml/min;柱温30℃;进样量20μl。结果成功分离出虎杖饮片提取液中的虎杖苷、白藜芦醇、大黄素、大黄素甲醚等有效成分。结论该方法稳定有效,具有良好的耐用性,对虎杖饮片的数据识别具有指导意义。Objective To study the fingerprint of Polygonum cuspidatum from various regions by HPLC and provide technical support for the identification.Methods HPLC conditions：the chromatographic column was Agilent ZORBAX SB-C18（4.6mm×250mm,5μm）;the mobile phase was 0.1%formic acid in water and acetonitrile（A-B）;gradient elution;the detection wavelength was 306nm;the flow rate was 1ml/min;the column temperature was 30 ℃;injection volume was 20μl.Results The HPLC gradient conditions could effectively separate polydatin,resveratrol,emodin and emodin ether,etc.Conclusion This method is stable,effective and durable,which is instructive to the herbs data identification of Polygonum cuspidatum.

关 键 词：虎杖 中药饮片 高效液相色谱法 指纹图谱 耐用性 

分 类 号：R93[医药卫生—生药学]