甘草黄酮对UVB损伤HDF细胞保护作用研究  

Effects of Licoflavone on HDF Cytoprotection after UVB Injury

作　　者：付维维[1,2] 刘娟[1] 王业秋[2] 李艳微[2] 徐艳明[2] 张宁[2] 李建民[2] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154007 [2]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《安徽农业科学》2016年第4期170-171,174,共3页Journal of Anhui Agricultural Sciences

基　　金：黑龙江省应用技术研究与开发计划项目(PC13S15)

摘　　要：[目的]研究甘草黄酮对UVB照射后产生光老化现象的HDF细胞保护作用。[方法]采用30 m J/cm^2的UVB照射HDF细胞建立损伤模型;加入不同浓度的甘草黄酮,24 h后MTT法检测细胞活性;用试剂盒法测定细胞上清液中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量。[结果]甘草黄酮浓度为10^(-9)、10^(-10)、10^(-11)mol/L时,能使受UVB照射损伤的HDF细胞活性增强;甘草黄酮浓度为10^(-9)、10^(-10)mol/L时,GSH-Px、SOD活性升高,MDA含量降低;甘草黄酮浓度为10^(-11)mol/L时,SOD活性升高、MDA含量降低。[结论]甘草黄酮能减轻UVB对HDF细胞的损伤,其机制可能与抑制氧化损伤、提高SOD活性有关。[Objective] To research the effects of licoflavone on HDF cytoprotection after UVB injury. [Method] HDF was irradiated under 30 m J / cm^2 UVB to establish injury mode. After adding different concentrations of licoflavone,cell viability was detected by MTT method after 24 h.Contents of GSH-Px,SOD and MDA in cell supernatant were detected by kit method. [Result]When licoflavone concentration was 10^（-9）,10^（-10） and 10^（-11） mol / L,HDF activities enhanced after UVB injury. When licoflavone content was 10^（-9）and 10^（-10） mol / L,contents of GSH-Px,SOD and MDA reduced. When licoflavone content was 10^（-11） mol / L,SOD activity enhanced and MDA content reduced. [Conclusion]Licoflavone reduces the HDF injury induced by UVB. Its mechanism might be related to the restriction of oxidative damage and the enhancement of SOD.

关 键 词：甘草黄酮 光老化 UVB HDF细胞 保护作用 

分 类 号：S567.71[农业科学—中草药栽培]