丹参酮ⅡA对脑缺血再灌注大鼠顶叶皮质TNF-α和NF-κB表达的影响  

Effect of tanshinone IIA on the expressions of TNF-α and NF-κB in parietal cortex of cerebral ischemia-reperfusion rats

作　　者：梁鑫[1] 夏春义[1] 陈荣举 傅志坚[1] 

机构地区：[1]沈阳市第一人民医院神经外科,辽宁沈阳110041

出　　处：《解剖科学进展》2016年第2期152-154,共3页Progress of Anatomical Sciences

基　　金：辽宁省科技攻关资助项目(201225020)

摘　　要：目的探讨丹参酮ⅡA对局灶性脑缺血再灌注大鼠顶叶皮质肿瘤坏死因子-α(TNF-α)和核转录因子-κB(NF-κB)表达的影响。方法选取36只健康雄性大鼠,随机分为假手术组(Sham,12只),缺血再灌注组(I/R,12只)和丹参酮ⅡA治疗组(TanⅡA,12只),用线栓法阻塞大鼠右大脑中动脉制作局灶性脑缺血再灌注模型,脑缺血2h再灌注24h后,应用免疫组织化学方法与Western blot方法检测大鼠手术侧顶叶皮质TNF-α和NF-κB的表达。结果与Sham组相比,I/R组大鼠顶叶皮质TNF-α与NF-κB蛋白的阳性表达的平均光密度值显著升高(<0.05);而与I/R组相比,TanⅡA组大鼠顶叶皮质TNF-α与NF-κB蛋白的阳性表达的平均光密度值显著降低(<0.05)。结论丹参酮ⅡA能够降低脑缺血再灌注大鼠顶叶皮质TNF-α与NF-κB蛋白的表达水平。Objective To study the effect of tanshinone IIA on the expressions of tumor necrosis factor（TNF）-α and nuclear factor（NF）-κB in parietal cortex of cerebral ischemia-reperfusion rats.Methods 36 healthy male rats were randomly divided into sham group（12）,ischemia-reperfusion group（I/R,12）group,and tanshinone IIA treatment group（Tan ⅡA,12）.The focal cerebral ischemia reperfusion model was induced by occluding the right middle cerebral artery using the intraluminal suture method.The expressions of TNF-α and NF-κB were detected by immunohistochemistry and Western blot 2h after occlusion following with 24 h reperfusion.Results Compared with sham group,the expression levels of TNF-α and NF-κB in parietal cortex of rats were increased significantly in I/R group（p〈0.05）;Compared with I/R group,the expression levels of TNF-α and NF-κB in parietal cortex of rats were decreased significantly in Tan ⅡA group（p〈0.05）.Conclusion Tan ⅡA could down-regulate the expressions of TNF-αand NF-κB in parietal cortex of cerebral ischemia-reperfusion rats.

关 键 词：脑缺血再灌注 丹参酮ⅡA 肿瘤坏死因子-Α 核转录因子-ΚB 大鼠 

分 类 号：R651.1[医药卫生—外科学]