锌离子对TGF-β1诱导的肾小管上皮细胞转分化的影响及机制  

Zinc protects rat kidney tubular epithelial cells from undergoing TGF-β1-induced epithelial-to-mesenchymal transition

作　　者：姚璐[1] 张秀丽[2,3] 赵越[2] 张萍[4] 王雪梅[5] 池志宏[6] 

机构地区：[1]中国医科大学科学实验中心,沈阳110122 [2]中国医科大学细胞生物国家重点实验室,沈阳110122 [3]本溪市中心医院肾内科,本溪117000 [4]辽宁医学院组胚教研室,锦州121000 [5]中国医科大学附属口腔医院修复科,沈阳110001 [6]中国医科大学病理生理教研室,沈阳110122

出　　处：《解剖科学进展》2016年第2期186-189,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81170561);辽宁省自然科技计划基金(2012408002;2013225090);中国博士后科学基金(2014MM551144)

摘　　要：目的研究锌离子对转化生长因子-β1(TGF-β1)诱导的肾小管上皮细胞转分化(EMT)的影响。方法常规培养肾小管上皮细胞、传代、分组:①正常对照组;②TGF-β1组(10ng/ml TGF-β1作用48h);③ZnSO_4作用组(30μM ZnSO_4作用24h后,加10ng/ml TGF-β1作用48h)。采用相差显微镜观察各组细胞表型改变,免疫荧光及Western blotting方法检测波形蛋白(vimentin)、上皮钙黏素(E-cadherin)、Ⅰ型胶原酶(collagenⅠ)蛋白表达。应用Western blotting检测p-Akt蛋白表达。结果 TGF-β1可以导致肾小管上皮细胞vimentin、collagenⅠ和p-Akt蛋白表达增高,使E-cadherin蛋白表达降低。ZnSO_4作用后可有效降低由TGF-β1导致的肾小管上皮细胞vimentin、collagenⅠ及p-Akt的高表达,同时使E-cadherin蛋白表达增多。结论 ZnSO_4阻抑TGF-β1所致的肾小管上皮细胞的EMT,其作用可能与PI-3K/Akt信号通路相关。Objective To investigate that the effect of Zn^2＋ on transforming growth factor-β1（TGF-β1）-induced epithelial-to-mesenchymal transition（EMT）in rat kidney tubular epithelial cells（NRK-52 E cells）.Methods NRK-52 E cells were cultured in a 5% CO2 atmosphere in complete Dulbecco＇s modified Eagle＇s medium（DMEM;low glucose）and the cells were classified into the following three groups：normal control group,TGF-β1 group（10ng/ml TGF-β1 treatment for 48 hours）,TGF-β1/ZnSO4 group（30μM ZnSO4 sulfate pre-treatment followed by 10ng/ml TGF-β1）.The expression of vimentin,E-cadherin,collagen I and P-Akt was detected by immunofluorescence staining or Western blotting.Results TGF-β1 significantly increased the expression levels of vimentin and collagen I and p-Akt,but ameliorated expression level of E-cadherin in NRK-52 E cells.However,pretreatment of Zn SO4 downregulaed TGF-β1-induced overexpression of vimentin and collagen I and p-Akt,and upregulated TGF-β1-induced downexpression of E-cadherin in NRK-52 E cells.Conclusion The inhibition of Zn SO4 on EMT in TGF-β1-induced NRK-52 E cells might be related to PI-3K/Akt pathways.

关 键 词：TGF-Β1 ZnSO4 转分化 糖尿病肾病 肾小管上皮细胞 

分 类 号：Q254[生物学—细胞生物学]