糖皮质激素受体mRNA在阻塞性睡眠呼吸暂停低通气综合征患者外周血单个核细胞中的表达  

Glucocorticoid receptors mRNA expression in peripheral blood mononuclear cell in patients with obstructive sleep apnea hypopnea syndrome

作　　者：何丰[1] 黎景佳[1] 常利红[1] 黄肇通[1] 王志远[1] 李培[1] 王海峰[1] 叶进[1] 

机构地区：[1]中山大学附属第三医院耳鼻咽喉头颈外科,广东广州510630

出　　处：《中国耳鼻咽喉头颈外科》2016年第3期159-162,共4页Chinese Archives of Otolaryngology-Head and Neck Surgery

摘　　要：目的探讨阻塞性睡眠呼吸暂停低通气综合征(OSAHS)患者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中糖皮质激素受体(glucocorticoid receptor,GR)mRNA的表达及其意义。方法采用实时荧光定量PCR检测30例中重度男性OSAHS患者和27例正常男性PBMC中GRαmRNA和GRβmRNA的表达,探讨其与呼吸暂停低通气指数、体质量指数、颈围、腰围、最低动脉血氧饱和度、平均动脉血氧饱和度、Epworth嗜睡量表评分、空腹血糖、心率及血压之间的关系。结果 OSAHS组的GRαmRNA表达水i砰较对照组低(t=2.25,P<0.05);GRβmRNA的表达水平在两组间无显著差异(t=1.43,P>0.05)。在OSAHS组中并未发现GRαmRNA的表达与各参数之间存在显著相关性。结论中重度男性OSAHS患者PBMC中GRαmRNA的表达水平较对照组下降,这种改变的机制目前尚不清楚。OBJECTIVE To study the mRNA expression of glucocorticoid receptor（GR） in peripheral blood mononuclear cells in patients with obstructive sleep apnea hypopnea syndrome（OSAHS） and its clinical significance.METHODS Real-time fluorescent quantitative PCR was used to detect GRα mRNA and GRβ mRNA exprssion in PBMC of 30 male patients with moderate to severe OSAHS and 27 healthy male subjects.The relationships between the expression of glucocorticoid receptor mRNA and the apnea hypopnea index,body mass index,neck circumference,waist circumference,the lowest oxygen saturation,the average oxygen saturation,the Epworth sleepiness scale（ESS） score,fasting blood glucose,heart rate and blood pressure were analyzed.RESULTS Compared with the control group,the expression level of GRα mRNA was lower in the OSAHS group（t=2.25,P〈0.05） and the expression level of GRβmRNA had no difference between the two groups（t=1.43,P〉0.05）.We had not found the significant correlation between the expression of GRα mRNA and the clinical parameters above in OSAHS patients.CONCLUSION The expression of GRα mRNA in PBMC in moderate to severe OSAHS male patients have a downward trend compared with healthy group,but the mechanism remains unclear.

关 键 词：睡眠呼吸暂停 阻塞性 受体 糖皮质激素 外周血单个核细胞 

分 类 号：R766[医药卫生—耳鼻咽喉科]