Simple colorimetric detection of dopamine using modified silver nanoparticles  

作　　者：Sivakumar Palanisamy Xuehua Zhang Tao He 

机构地区：[1]Key Laboratory of Nanosystem and Hierarchical Fabrication,Chinese Academy of Sciences,National Center for Nanoscience and Technology,Beijing 100190,China [2]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China Chemistry》2016年第4期387-393,共7页中国科学（化学英文版）

基　　金：supported by the National Basic Research Program of China(2011CB933200)

摘　　要：Dopamine(DA) plays an important role in health and peripheral nervous systems. Colorimetric detection of DA has the advantage of color change and simplicity in operation and instrumentation. Herein, we report a highly sensitive and selective colorimetric detection of DA by using two specific ligands modified Ag nanoparticles, where the DA molecules can make dual recognition with high specificity. The colloidal suspension of modified Ag nanoparticles was agglomerated after interacting with DA, while the color of Ag nanoparticles suspension changed from yellow to brown, arising from the interparticle plasmon coupling during the aggregation of Ag nanoparticles. The modified Ag nanoparticles suspension and agglomeration were confirmed by transmission electron microscope images. The optical properties behind the color change were thoroughly investigated by using UV-Vis and Raman techniques. The changes in p H, zeta potential, particle size and surface charge density by adding DA were also determined by using dynamic light scattering measurements. The detection limits of modified Ag probes for DA was calculated to be 6.13′10^(-6) mol L^(-1)(S/N=2.04) and the correlation co-efficient was determined to be 0.9878. Because of the simplicity in operation and instrumentation of the colorimetric method, this work may afford a feasible, fast approach for detecting and monitoring the DA levels in physiological and pathological systems.Dopamine （DA） plays an important role in health and peripheral nervous systems. Colorimetric detection of DA has the ad- vantage of color change and simplicity in operation and instrumentation. Herein, we report a highly sensitive and selective colorimetric detection of DA by using two specific ligands modified Ag nanoparticles, where the DA molecules can make dual recognition with high specificity. The colloidal suspension of modified Ag nanoparticles was agglomerated after interacting with DA, while the color of Ag nanoparticles suspension changed from yellow to brown, arising from the interparticle plasmon coupling during the aggregation of Ag nanoparticles. The modified Ag nanoparticles suspension and agglomeration were con- firmed by transmission electron microscope images. The optical properties behind the color change were thoroughly investi- gated by using UV-Vis and Raman techniques. The changes in pH, zeta potential, particle size and surface charge density by adding DA were also determined by using dynamic light scattering measurements. The detection limits of modified Ag probes for DA was calculated to be 6.13×10 6 mol L-1 （S/N=2.04） and the correlation co-efficient was determined to be 0.9878. Be- cause of the simplicity in operation and instrumentation of the colorimetric method, this work may afford a feasible, fast ap- proach for detecting and monitoring the DA levels in physiological and pathological systems.

关 键 词：dopamine detection Ag nanoprobe COLORIMETRY dual molecular recognition sensitivity SELECTIVITY 

分 类 号：R446[医药卫生—诊断学] O657.3[医药卫生—临床医学]