Colorimetric detection and efficient monitoring of a potential biomarker of lumbar disc herniation using carbon nanotube-based probe  

作　　者：Yufeng Huang Ju Liu Min Zou Qingzhi Zhang Jie Chao Weidong Zhao Desheng Wu Shao Su Lianhui Wang 

机构地区：[1]Department of Spine Surgery,Shanghai East Hospital,Tongji University,Shanghai 200120,China [2]Department of Orthopedic Surgery,Shanghai Pudong New District Zhoupu Hospital,Shanghai 201318,China [3]Key Laboratory for Organic Electronics&Information Displays,Institute of Advanced Materials,National Syngerstic Innovation Center for Advanced Materials,Nanjing University of Posts&Telecommunications,Nanjing 210023,China

出　　处：《Science China Chemistry》2016年第4期493-496,共4页中国科学（化学英文版）

基　　金：supported by the National Basic Research Program of China(2012CB933301);the Key Discipline Construction Project of Pudong Health Bureau of Shanghai(PWZx2014-02);the Sci-tech Support Plan of Jiangsu Province(BE2014719);the Priority Academic Program Development of Jiangsu Higher Education Institutions

摘　　要：Herein, a multiwalled carbon nanotubes(MWNT)-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1(Apo L1) in lumbar disc herniation(LDH) patients. Apo L1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group(Ctrl), spine spondylolisthesis(SSP) group, spinal fracture(SFR) group, and spine scoliosis(SSC) group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was <0.001 and >0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay(ELISA), respectively, suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that Apo L1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a potential application in discovering new biomarkers of diseases.Herein, a multiwalled carbon nanotubes （MWNT）-based colorimetric probe was designed to discover and monitor the level of apolipoprotein-L1 （ApoL1） in lumbar disc herniation （LDH） patients. ApoL1 could be easily found in human serum of the LDH group, but not obviously expressed in the normal control group （Ctrl）, spine spondylolisthesis （SSP） group, spinal frac- ture （SFR） group, and spine scoliosis （SSC） group. Furthermore, the as-prepared MWNT-based probe was also used to track the recovery of LDH patients who have successful surgery operation. The P value of early diagnosis and recovery monitoring was 〈0.001 and 〉0.05 for the proposed method and a conventional enzyme-linked immunosorbent assay （ELISA）, respectively suggesting this detection strategy had significant differences compared with the traditional ELISA. All experimental results showed that ApoL1 might be a potential biomarker for early diagnosis of LDH. This proposed detection strategy has a poten- tial application in discovering new biomarkers of diseases.

关 键 词：colorimetric probe apolipoprotein-L1 early diagnosis recovery monitoring 

分 类 号：O657.3[理学—分析化学] R681.53[理学—化学]