伯氏疟原虫哌喹抗性系小鼠病理学特征与抗性相关性研究  

Pathological features and resistant correlation of the piperaquine resistant line mice infected with Plasmodium berghei

作　　者：黄宪希[1] 周利民[1] 沈笑[1] 易国辉[1] 薛伟玲[1] 郭虹[1] 

机构地区：[1]海南医学院科学实验中心,海南海口571199

出　　处：《中国热带医学》2016年第3期197-202,共6页China Tropical Medicine

基　　金：国家自然科学基金(No.81060140);海南医学院科研启动基金(No.2011011)

摘　　要：目的观察伯氏疟原虫(Pb)ANKA株哌喹抗性系(PQR)感染小鼠组织病理学变化特征,比较与哌喹敏感系(PQS)的差异,探讨其抗性机制。方法实验小鼠随机分为A(PQS)、B(PQR)及C(正常对照,NC)三组,取肝、脾、脑及肾组织,10%福尔马林固定后石蜡包埋,4μm切片,苏木精一伊红(Haematoxylin-eosin,HE)及吉姆萨(Giemsa)染色,镜下观察其组织病理学改变。结果与PQS组比较,PQR组疟原虫感染红细胞(p RBC)及疟色素(HZ)在各器官组织中出现晚,增殖缓慢,数量明显偏少,在17d增至峰值时仍明显少于PQS组6d时,22d时开始下降。各组织的炎性病变随感染天数的延长而变化:早期较轻,呈渐进性发展,17d时达到高峰,22d时炎症减轻,组织损伤呈恢复趋势,其两组肝、脾组织中炎性细胞的浸润程度在6d时未见明显不同。脑组织的炎性反应、疟原虫浸润及组织损伤程度均显著轻于PQS组。结论 PQR系的毒力下降、致病力降低表象的组织病理学特征为:肝、脾、脑和肾组织中p RBC及HZ浸润量明显偏低,炎性反应、原虫数量的变化呈逐渐增加、降低、趋向恢复的动态过程,未见明显的脑型疟的组织学变化。Objective To explore the mechanism of piperaquine resistance（PQR） by evaluating and comparinghistopathological features of mice infected with PQR line and the piperaquine sensitive（PQS） line of Plasmodium berghei（Pb）ANKA strain. Methods Mice were randomly divided into A（PQS）, B（PQR） and C（normal control, NC） groups. Tissuesections of the liver, spleen, brain and kidneys were collected, fixed in 10% Formalin, embedded in paraffin wax, cut into 4 μmthick slices, and then stained by Haematoxylin-eosin（HE） and Giemsa. The histopathological changes were observed by a lightmicroscope. Results In comparison to PQS group, parasitised Red Blood Cells（p RBCs）and malaria pigments hemozoin（HZ）in PQR group appeared later, increased slower, and had less quantity in all tissues. The quantity stayed significantly less inPQR group even when reaching the peak at day 17 post-infection（pi） compared with that in PQS group at day 6 pi, and beganto drop at day 22 pi. The inflammatory change in all tissues varied with infection days elapsed. Inflammatory reactions wereslight at the early stage, progressed gradually and reached the peaks at day 17 pi. Starting from day 22, the inflammationreactions reduced and the tissue damage recovered. There was no visible difference in inflammatory cell infiltration in liver andspleen at day 6 pi between two groups. The degrees of inflammation, protozoa infiltration and tissue damages in the brain wereobviously milder in PQR group than PQS group. Conclusion With the reduced virulence and pathogenicity, the PQR linedisplayed lower infiltration of p RBC and HZ compared to PQS line in histopathology. The inflammatory responses andquantities of protozoa and HZ changed dynamically with a gradual increase followed by a reduction and then recovery. Therewere no distinct histological changes of cerebral malaria observed.

关 键 词：伯氏疟原虫 哌喹抗性 疟色素 组织病理学 苏木精-伊红染色法 

分 类 号：R531.3[医药卫生—内科学]