新疆紫草中左旋紫草素的快速制备  

Separation and isolation of shikonin from Arnebia Euchroma Johnst by flash chromatography

作　　者：申刚义[1] 李书文[1] 张爱芹[2] 刘佳[1] 张蕾[1] 

机构地区：[1]中央民族大学中国少数民族传统医学国家民委-教育部重点实验室,北京100081 [2]中央民族大学生命与环境科学学院,北京100081

出　　处：《分析试验室》2016年第3期277-279,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(81573834);国家民委科研项目(14ZYZ018);国家科技支撑计划项目(2014BAC15B04)资助

摘　　要：利用快速制备色谱技术,首次开展了从新疆紫草中分离制备左旋紫草素的研究。重点考察了洗脱液、流速、进样量对分离制备的影响。最终选用甲醇-水为两相溶剂系统（80%～100%,V/V）,流速为5 m L/min,进样量为10 m L进行分离制备。结果显示40 min内制备得到3种有效成分。所得产物纯度经毛细管电泳检测,结构经NMR确证分别为左旋紫草素、乙酰紫草素和β-羟基异戊酰紫草素3种单体。实际可从10 m L萘醌类提取物中制备左旋紫草素15 mg。该方法为快速制备天然产物的活性成分提供了新手段。A flash chromatography method for fast isolating shikonin from Arnebia Euchroma Johnst was developed. The preparation conditions such as mobile phase system,the rate of mobile phase and injection volume of sample were investigated. The optimizing separation system was constructed with a two-phase solvent system composed of methanol-water（ 80% ～ 100%,V / V） at a flow rate of 5. 0 m L / min and the injection volume of sample with 10 m L. The results indicated that 3 kinds of compositions could be well separated and obtained within 40 mins. The obtained fractions were analyzed by capillary electrophoresis and identified by NMR. 15 mg shikonin was obtained from 100 mg crude extract by fast separations. It was expected that the method would be a powerful technique of purification for natural products.

关 键 词：新疆紫草 左旋紫草素 快速制备色谱 

分 类 号：R284.2[医药卫生—中药学] O657.7[医药卫生—中医学]