血清ADA水平和T-spot.TB联合检测在肺结核诊断中的应用  

Application of combined detection of serum levels of ADA and T-spot.TB in diagnosis of pulmonary tuberculosis

作　　者：冯晓朦 王晶莹[1] 时景伟[1] 李威[1] 高申 冯雪飞[1] 谢风[1] 

机构地区：[1]吉林大学中日联谊医院检验科,吉林长春130033

出　　处：《吉林大学学报（医学版）》2016年第2期306-310,共5页Journal of Jilin University：Medicine Edition

基　　金：国家"863"计划资助子项目资助课题(20141422010)

摘　　要：目的:联合应用血清腺苷脱氨酶(ADA)水平检测和结核感染T细胞斑点试验(T-spot.TB),探讨其对肺结核诊断的临床价值。方法:选取本院拟诊为肺结核患者159例,分为肺结核组(n=91)和非肺结核组(n=68),随机选取健康体检者80名作为健康对照组,检测各组受试者血清ADA水平和T-spot.TB斑点数,比较各组ADA水平和T-spot.TB的阳性率及其对肺结核诊断的灵敏性与特异性。结果:肺结核组、非肺结核组和健康对照组受试者血清ADA水平分别为(22.10±6.60)、(16.90±6.35)和(8.70±5.98)U·L^(-1),肺结核组患者血清ADA水平明显高于非肺结核组和健康对照组(P<0.05)。血清ADA水平对肺结核诊断的阳性率为56.0%,T-spot.TB对肺结核诊断的阳性率为87.9%。采用平行联合试验,检测灵敏性为91.2%;采用系列联合试验,特异性为94.6%。结论:联合检测血清ADA水平及T-spot.TB斑点数能够显著提高肺结核临床诊断。Objective： To combine the detection of serum levels of adenosine deaminase （ADA） and T-cell spot test （T-spot. TB）, and to explore their significances in diagnosis of pulmonary tuberculosis. Methods： 159 patients suspected with pulmonary tuberculosis were selected and divided into pulmonary tuberculosis group and non- tuberculosis group （n=68）; 80 healthy people were randonly selected as heahhy control group. The serum ADA levels and number of T-spot of the subjects in three groups were detected. Ther serum AI）A levels and the positive rates of T-spot. TB in various groups and their sensitivities and specifities were compared. Results： The serum ADA level of the patients in pulmonary tuberculosis gruop was （22.10±6.60） U · L1 ; those in non-tuberculosis group and healthy control group were （16.90±6.35） and （8.70±5.98） U · L -1 the serum ADA level in pulmonary tuberculosis group was significantly higher than those in non-tuberculosis group and heathy control group （P〈0.05）. The positive rate of serum ADA level in diagnosis of pulmonary tuberculosis was 56~ and the T- spot. TB positive rate in diagnosis of pulmonary tuberculosis was 87.9%. Combined use of parallel test, the detection sensitivity was 91.2%; using the series of joint tests, the specificity was 94.6%. Conclusion： Combined detection of serum level of ADA and T-spot. TB can significantly improve the clinical diagnosis efficacy of pulmonary tuberculosis.

关 键 词：肺结核 腺苷的脱氨酶 结核感染T细胞斑点试验 

分 类 号：R52[医药卫生—内科学]