N-乙酰半胱氨酸对糖尿病大鼠肺组织氧化应激及p22phox表达的影响  

Effects of N-acetylcysteine on oxidative stress and the expression of p22phox in lung tissue of diabetic rats

作　　者：张元[1] 夏中元[1] 苏娃婷[1] 雷少青[1] 

机构地区：[1]武汉大学人民医院麻醉科,430060

出　　处：《中华糖尿病杂志》2016年第3期168-171,共4页CHINESE JOURNAL OF DIABETES MELLITUS

基　　金：国家自然科学基金（81300674）

摘　　要：目的观察糖尿病大鼠肺组织氧化应激和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶膜结合亚基p22phox表达变化及抗氧化剂N-乙酰半胱氨酸（NAC）对其影响。方法18只雄性SD大鼠（220～250g），采用随机数字表法分为正常对照组（C组，6只）、糖尿病组（D组，6只）和糖尿病NAC治疗组（D＋N组，6只）。4周后，获取血浆及肺组织，试剂盒检测游离异前列腺素、总抗氧化物含量、总超氧化物歧化酶（SOD）活性以及过氧化氢酶（CAT）活性。Western blotting检测p22phox蛋白表达情况。两两比较采用t检验，组间比较采用单因素方差分析。结果与C组相比，D组大鼠肺组织p22phox蛋白表达量显著增加（1．25±0．18比1．00±0．00，t=3．39，P〈0．05），血浆与肺组织游离异前列腺素含量、血浆总抗氧化物含量显著增加（t=14．39、8．48、14．72，均P〈0．05），而肺组织总抗氧化物活性、血浆与肺组织总SOD活性显著降低（t=22．70，12．64、11．39，均P〈0．05）。经NAC治疗4周后，肺组织p22phox、总抗氧化物活性、异前列腺素、总SOD均较D组明显改善[分别为1．01±0．14比1．25±0．18、（0．69±0．06）比（0．41±0．03）mmol／g、（390±21）比（505±40）pg／mg、（2．22±0．12）比（1．84±0．05）U／rag，t=10．54、3．19、2．56、6．26，均P〈0．051，但肺组织CAT活性却显著降低（t=8．65，均P〈0．05）。结论NAC能改善下调糖尿病大鼠血浆及肺组织氧化应激状态，同时下调肺组织p22phox蛋白表达水平。Objective To observe the changes of oxidative stress and the expression of p22phox,a membrane subunit of Nicotinamide Adenine Dinucleotide Phosphate-oxidase（NADPH）, and effects of the antioxidant N-acetylcysteine（NAC） in the lung tissue of diabetic rats. Methods A total of 18 male SD rats （220-250 g） were randomly divided into normal control group （group C, n=6）, diabetic group （group D, n=6）, and diabetes wittl NAC treated group （group （D＋N）i n=6） by random digital table method. After 4 weeks, the plasma and lung tissue of rats were collected for detection of the total antioxidant, free 15-F2t-isoprostane, total superoxide dismutase（SOD） activity and Catalase（CAT） activity by kits, and p22phox protein expression by Western blotting. Results Compared with C group rats,the levels of p22phox expression in lung tissues in diabetic rats significantly increased （1.25±0.18 vs 1.00±0.00, t=3.39, P〈0.05）.The levels of 15-F2t- isoprostane in plasma and lung tissue and total antioxidant concentration in diabetic rats significantly increased（t=14.39, 8.48, 14.72,all P〈0.05） as compared with that in C rats, but the total antioxidant concentration in lung tissue, and total SOD activity and CAT activity in plasma and lung tissue significantly decreased（t=22.70, 12.64, 11.39, all P〈0.05）.The levels of p22phox, total antioxidant, free 15-F2t- isoprostane,total superoxide dismutasein lung tissue by NAC treatment for 4 weeks were significantly improved than that of D group （1.01±0.14 vs 1.25±0.18, （0.69±0.06）vs （0.41 ±0.03）mmol/g, （390±21）vs （505±40） pg/mg, （2.22±0.12） vs （1.84±0.05） U/mg;t=10.54,3.19,2.56,6.26,all P〈0.05）. All these changes were reversed or attenuated （t=8.90,6.23,2.58,6.26,10.54,1.81,7.19,all P〈0.05）remarkably by NAC treatment for 4 weeks, while the CAT activity in lung tissue unexpectedly declined （t=8.65, all P〈0.05）. Conclusions NAC can attenuate oxidative stress in plasma and lung tissue of diabetic ra

关 键 词：N-乙酰半胱氨酸 糖尿病肺 氧化应激 烟酰胺腺嘌呤二核苷酸磷酸氧化酶 P22PHOX 

分 类 号：R587.1[医药卫生—内分泌]