蜈蚣提取物抗肺癌活性的体内外实验研究  

Study on Anti-lung Cancer Efficiency of Centipede Extracts in Vitro and Vivo Experiments

作　　者：陈园[1] 艾小佳[1] 王志琪[1] 田莎[1] 周青[2] 裴刚[1] 田雪飞[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学第一附属医院,湖南长沙410007

出　　处：《中国中医药信息杂志》2016年第5期61-63,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81473617);湖南省自然科学基金重点项目(13JJ2032);湖南省教育厅重点资助项目(10A089)

摘　　要：目的探讨蜈蚣提取物对人肺癌A549细胞的增殖抑制、凋亡诱导及裸小鼠皮下移植瘤生长的抑制作用。方法采用酶解法合丙酮沉淀法分离纯化蜈蚣提取物处理人肺癌A549细胞,MTT法检测细胞增殖抑制并计算半数抑制率(IC50),流式细胞术检测细胞凋亡和细胞周期变化,Hoechst荧光染色观察细胞凋亡的形态变化。制备人肺癌细胞裸小鼠皮下移植瘤模型,随机分为模型组、对照组和蜈蚣提取物组,每组10只,观察裸小鼠皮下移植瘤瘤体体积、质量及抑瘤率变化。结果体外实验结果显示,蜈蚣提取物对人肺癌A549细胞有明显的增殖抑制作用,IC50为0.603 mg/m L;蜈蚣提取物作用48 h后,细胞周期表现为G0/G1期细胞比例下降,S期与G2/M期细胞比例均升高,与对照组比较差异有统计学意义(P<0.01);Hoechst荧光染色可观察到明显的凋亡细胞及凋亡特征性的形态学改变;动物体内抑瘤实验结果发现,经蜈蚣提取物治疗后,瘤体质量与体积显著减少,与模型组比较差异有统计学意义(P<0.01)。结论蜈蚣提取物对人肺癌A549细胞具有浓度依赖性增殖抑制作用,可诱导细胞凋亡,使A549细胞停滞于G2/M期,并能有效抑制人肺癌裸小鼠皮下移植瘤的生长。Objective To investigate efficiency of centipede extracts on apoptosis induction, proliferation inhibition to Human A549 cell line and growth suppression of subcutaneous transplanted sarcoma in nude mice. Methods Centipede extracts prepared by enzymolysis and acetone precipitation methods were used to treat human lung cancer A549 cell line. Proliferation inhibition was evaluated by MTT assay and half inhibit concentration (IC50) was calculated. Cell morphological change and apoptosis were detected by flow cytometry and Hoechst stain. The subcutaneous transplanted sarcoma models were prepared with nude mice and randomly divided into model group, control group and centipede extracts group, with 10 mice in each group. Changes of tumor volume, quality and anti-tumor rate were observed.Results In vitro experiment, proliferation of A549 cells was inhibited with dose-dependency and IC50 value was 0.603 mg/mL. The G0/G1 phase of cells was down regulated and G2/M and S phase cells were up-regulated. The apoptotic character cells were been found by Hoechst stain. In vivo experiment, the tumor weight and volume decreased significantly compared with model control group, with statistical significance (P<0.01).Conclusion The centipede extracts shows dose-dependent anti-proliferative effect on A549 cells, which can induce apoptosis by arresting A549 cells at G2/M phase and suppressing growth of subcutaneous transplanted sarcoma of lung cancer in nude mice.

关 键 词：蜈蚣提取物 肺癌 细胞凋亡 移植瘤 小鼠 

分 类 号：R285.5[医药卫生—中药学]