RP-HPLC法同时测定芍枣胶囊中3种主要成分的含量  

Determination of three main constituents in Shaozao capsules by RP-HPLC

作　　者：王羽凝[1] 穆丽华[2] 王石 魏沛沛 王茹[5] 刘屏[2] 

机构地区：[1]解放军总医院外科临床部,北京100853 [2]解放军总医院临床药理研究室,北京100853 [3]解放军医学院,北京100853 [4]蚌埠医学院,安徽蚌埠233003 [5]解放军总医院门诊药房,北京100853

出　　处：《中国药物应用与监测》2016年第2期77-79,84,共4页Chinese Journal of Drug Application and Monitoring

基　　金：军队"十二五"中医药推广研发项目(10ZYZ141)

摘　　要：目的：建立同时测定芍枣胶囊中3种主要成分的RP-HPLC方法。方法：采用RP-HPLC法,选用Kromasil C18色谱柱（4.6 mm×250 mm,5μm）,流动相：乙腈（A）和水（B）;洗脱梯度：0~10 min 10%~19%A,10~16 min 19%~20%A,16~22 min20%~90%A,22~25 min 90%A,25~25.1 min 90%~10%A,25.1~35 min 10%A;流速：0.8 m L·min-1。Alltech 2000ES ELSD检测器,漂移管温度：115℃,空气流速3.3 L·min-1。结果：结果表明芍药苷,斯皮诺素,3,6＇-二芥子酰基蔗糖的检测分别在0.975~5.850μg,0.060~0.660μg,0.100~0.600μg范围内线性关系良好。上述3种成分的平均回收率（n=9）分别为99.5%（RSD 0.69%）,100.2%（RSD 1.03%）,99.9%（RSD 1.20%）。结论：该方法操作简单、结果准确,具有较好的重复性和稳定性,为芍枣胶囊的质量控制提供理论参考。Objective： To establish the quantitative method of three main constituents in Shaozao capsules by RPHPLCmethod. Methods： The separation was performed on Kromasil C18 column （4.6 mm × 250 mm, 5 μm） by RP-HPLCmethod. The mobile phase consisted of acetonitrile （A） and water （B）, elution gradient was as follows： 0 –10 min 10% –19% A; 10 – 16 min 19% – 20% A; 16 – 22 min 20% – 90% A; 22 – 25 min 90% A; 25 – 25.1 min 90% – 10% A; 25.1 –35 min 10% A. The flow rate was 0.8 mL·min-1. The detector was Alltech 2000ES ELSD. The temperature of drift tube was115 ℃ and the air flow rate was 3.3 L·min-1. Results： The linear ranges of the three components were 0.975 – 5.850 μg（paeoniflorin）, 0.060 – 0.660 μg （spinosin） and 0.100 – 0.600 μg （3,6＇-disinapoyl sucrose） respectively. The average recoveriesof the above three components in Shaozao capsules were 99.5% （RSD 0.69%）, 100.2% （RSD 1.03%）, 99.9% （RSD 1.20%）respectively. Conclusion： The method was reliable, simple, and precise, which was suitable for quality control of Shaozao capsules.

关 键 词：芍枣胶囊 芍药苷 斯皮诺素 3 6'-二芥子酰基蔗糖 反相高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]