CDK抑制剂SNS-032联合AD55-Apoptin对肝癌细胞Huh-7的体外抗癌作用  

In-vitro Antitumor Effect of Combining CDK Inhibitor SNS-032 with AD55-Apoptin on Liver Carcinoma Cell Huh-7

作　　者：孙笑竹[1] 吕春伟[1] 秦云[1] 韩建翠 王毅刚[1] 

机构地区：[1]浙江理工大学生命科学学院

出　　处：《浙江理工大学学报（自然科学版）》2016年第2期290-296,共7页Journal of Zhejiang Sci-Tech University(Natural Sciences)

基　　金：浙江省自然科学基金项目(LY13H080005);浙江省科技计划项目(2014C37101);浙江理工大学科研启动基金项目(14040365-J)

摘　　要：通过CDK抑制剂SNS-032联合AD55-Apoptin处理肝癌Huh-7细胞,以探究两者联合作用的体外抗癌作用。采用MTT法分别检测SNS-032、AD55-Apoptin以及SNS-032与AD55-Apoptin联合对肝癌细胞株Huh-7生长的抑制作用;利用Hoechst33342染色观察所处理细胞的凋亡形态学变化,采用流式细胞术对凋亡进行量化,Western Blot检测凋亡相关蛋白表达情况。结果表明：10MOI的AD55-Apoptin联合100ng/mL的SNS-032处理96h后,Huh-7细胞的存活率仅为4%,而10 MOI的AD55-Apoptin和100ng/mL SNS-032分别单独处理后细胞存活率为60%和8%（P〈0.05）;Hoechst33342染色、流式检测及Western Blot结果表明,联合处理组细胞凋亡现象更明显。实验结果证明SNS-032联合AD55-Apoptin能有效的抑制肝癌细胞Huh-7生长,并诱导其凋亡。CDK Inhibitor SNS-032 and AD55-Apoptin were combined to treat Huh-7to explore the invitro antitumor effect of the combination.MTT method was used to respectively detect inhibiting effects of SNS-032,AD55-Apoptin,the combination of SNS-032 and AD55-Apoptin on the growth of hepatoma cell line Huh7.The characteristic morphology of apoptosis cells were observed by Hoechst 33342 staining,flow cytometry was used to quantify the apoptosis,and Western Blot was used to detect the expression of apoptosis-related proteins.The results show that after treatment with the combination of 10 MOI AD55-Apoptin and 100ng/mL SNS-032,the Huh-7cell survival rate is only 4%,while in 10 MOI AD55-Apoptin and 100ng/mL SNS-032 separately treated cells,the survival rates are 60% and 8%（P 0.05）respectively.Hoechst 33342 staining,flow cytometry and Western Blot demonstrate that there are more apoptotic cells in the combined group.As shown in the experimental result,the combination of SNS-032 and AD55-Apoptin can effectively inhabit the growth of hepatoma carcinoma cell Huh-7and induce it to get apoptotic.

关 键 词：CDK抑制剂(SNS-032) AD55-Apoptin 肝癌 体外 抗癌作用 

分 类 号：Q26[生物学—细胞生物学]